Abstract
Steroid hormones exhibit diverse biological activities. Despite intensive studies on steroid function at the genomic level, their nongenomic actions remain an enigma. In this study, we investigated the role of reactive oxygen species (ROS) in androgen-stimulated prostate cancer (PCa) cell proliferation. In androgen-treated PCa cells, increased cell growth and ROS production correlated with elevated p66Shc protein, an authentic oxidase. This growth stimulation was blocked by antioxidants. Further, elevated expression of p66Shc protein by cDNA transfection encoding wild-type protein, but not a redox-deficient (W134F) mutant, was associated with increased PCa cell proliferation. Conversely, knockdown of p66Shc expression by shRNA resulted in diminished cell growth. Increased p66Shc expression in PCa cells enhanced their tumorigenicity in xenograft animals. Importantly, p66Shc protein level is higher in clinical prostate adenocarcinomas than in adjacent noncancerous cells. Expression of redox-deficient p66Shc mutant protein abolished androgen-stimulated cell growth. In androgen-treated, H(2)O(2)-treated, and p66Shc cDNA-transfected PCa cells, cellular prostatic acid phosphatase, an authentic tyrosine phosphatase, was inactivated by...Continue Reading
References
Dec 1, 1986·Molecular and Cellular Biology·M F LinG M Clinton
Jan 2, 1984·European Journal of Biochemistry·H C LiA Kirschonbaum
Feb 17, 1997·The EMBO Journal·E MigliaccioP G Pelicci
Aug 8, 1998·Breast Cancer Research and Treatment·L E Stevenson, A R Frackelton
Aug 15, 1998·The Journal of Biological Chemistry·T C Meng, M F Lin
Jul 6, 2000·The Prostate·T D OberleyL W Oberley
Nov 9, 2000·The Journal of Biological Chemistry·X Q ZhangM F Lin
Oct 19, 2001·Oncogene·K S Ravichandran
Feb 28, 2002·Molecular Cell·Tzu-Ching MengNicholas K Tonks
Apr 25, 2003·The Prostate·Tsukasa IgawaMing-Fong Lin
Oct 24, 2003·The Journal of Biological Chemistry·Andrea VenturaPier Giuseppe Pelicci
Dec 30, 2003·International Journal of Cancer. Journal International Du Cancer·Ming-Shyue LeeMing-Fong Lin
Mar 3, 2004·Oncogene·Ming-Shyue LeeMing-Fong Lin
Apr 14, 2004·The Journal of Biological Chemistry·Francesca OrsiniPier Giuseppe Pelicci
Jun 12, 2004·The Journal of Biological Chemistry·Tzu-Ching MengNicholas K Tonks
Sep 25, 2004·The Prostate·So Dug LimRebecca S Arnold
Feb 12, 2005·Endocrinology·Y J ParkD J Park
Jul 5, 2005·Mechanisms of Ageing and Development·S PandolfiC Franceschi
Jul 30, 2005·Cell·Marco GiorgioPier Giuseppe Pelicci
Sep 20, 2005·Oncogene·Suresh VeeramaniMing-Fong Lin
Dec 3, 2005·Endocrine-related Cancer·Suresh VeeramaniMing-Fong Lin
Feb 17, 2006·The Journal of Biological Chemistry·Shino NemotoToren Finkel
Mar 8, 2006·The Journal of Cell Biology·Firdous A KhandayKaikobad Irani
Oct 24, 2006·Nature Reviews. Molecular Cell Biology·Nicholas K Tonks
Dec 8, 2006·Cancer Letters·Miguel López-Lázaro
Jan 16, 2007·The Prostate·Siu-Ju ChenMing-Fong Lin
Oct 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Steven R GrossmanLaurie J Hafer
Dec 11, 2007·The FEBS Journal·Yi-Wei LouTzu-Ching Meng
Mar 29, 2008·Cancer Letters·J S ClerkinT G Cotter
May 28, 2008·Oncogene·S VeeramaniM-F Lin
Oct 23, 2008·Neuron·Mark J ZylkaPirkko Vihko
May 27, 2009·Trends in Biochemical Sciences·Suresh VeeramaniMing-Fong Lin
May 26, 2010·The Journal of Biological Chemistry·Tsai-Der ChuangMing-Fong Lin
Jan 26, 2011·PloS One·Santosh KumarMing-Fong Lin
Jan 28, 2012·Cancer Research·Laurent LessardMichel L Tremblay
Citations
Dec 20, 2012·Molecular Endocrinology·Yan ShiDaniel E Frigo
May 3, 2013·PloS One·Yu-Wei ChouMing-Fong Lin
Sep 17, 2013·PloS One·Ileana B QuinteroPirkko T Vihko
May 28, 2014·Oncogenesis·E TsoukoD E Frigo
Jul 23, 2014·Cancer Letters·Sakthivel MuniyanMing-Fong Lin
Apr 19, 2015·Molecular Cancer·Sahar S BhatFirdous A Khanday
Aug 11, 2015·Cellular Signalling·Sakthivel MuniyanMing-Fong Lin
Dec 20, 2014·European Journal of Clinical Investigation·Magdalena Lebiedzinska-ArciszewskaMariusz R Wieckowski
Apr 22, 2014·Biochimica Et Biophysica Acta·Sakthivel MuniyanMing-Fong Lin
Nov 5, 2013·Renal Failure·Shi-Kun YangLin Sun
Mar 7, 2013·Free Radical Biology & Medicine·Amélie RebillardJosiane Cillard
Jan 1, 2014·Free Radical Biology & Medicine·Jeroen FrijhoffArne Östman
Jan 8, 2014·Molecular Carcinogenesis·Sakthivel MuniyanMing-Fong Lin
Jul 12, 2019·Endocrine-related Cancer·Dannah R MillerMing-Fong Lin
Jan 11, 2014·The Journal of Biological Chemistry·Eric HainesAudrey Claing
Feb 14, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jiangyong YuJie Wang
Feb 21, 2018·Cellular Signalling·Matthew A IngersollMing-Fong Lin
May 18, 2019·Free Radical Biology & Medicine·Dannah R MillerMing-Fong Lin
Oct 17, 2020·BioMed Research International·Chenglin HanXunbo Jin