Reactive oxygen species involved in trichosanthin-induced apoptosis of human choriocarcinoma cells

The Biochemical Journal
C ZhangZ L Chen

Abstract

The type-I ribosome-inactivating protein trichosanthin (TCS) has a broad spectrum of biological and pharmacological activities, including abortifacient, anti-tumour and anti-HIV activities. We have found for the first time that TCS stimulated the production of reactive oxygen species (ROS) in JAR cells (a human choriocarcinoma cell line) in a time- and concentration-dependent manner by using the fluorescent probe 2',7'-dichlorofluorescein diacetate with confocal laser scanning microscopy. ESR spectral studies and the inhibition of ROS formation by the superoxide radical anion (O(2)(-.)) scavenger superoxide dismutase, the H(2)O(2) scavenger catalase and the hydroxyl radical (OH(.)) scavenger mannitol suggested the involvement of O(2)(-.), H(2)O(2) and OH(.). TCS-induced ROS formation was shown to be dependent on the presence of both extracellular and intracellular Ca(2+); moreover, ROS production paralleled the intracellular Ca(2+) elevation induced by TCS, suggesting that ROS production might be a consequence of Ca(2+) signalling. TCS-induced activation of caspase-3 was initiated within 2 h; however, TCS-induced production of ROS was initiated within 5 min, suggesting that the production of ROS preceded the activation of caspa...Continue Reading

References

Nov 25, 1991·Nucleic Acids Research·M X LiS I Chan
Jan 1, 1991·Annual Review of Cell Biology·R E EllisH R Horvitz
Feb 15, 1989·Archives of Biochemistry and Biophysics·D J McConkeyS Orrenius
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M S McGrathJ Marsh
May 4, 1986·Nature·X J Zhang, J H Wang
Sep 1, 1971·The Journal of Pathology·J F Kerr
May 24, 1995·Biochimica Et Biophysica Acta·B N AmesT M Hagen
Jan 27, 1995·Cell·D E Clapham
Feb 1, 1994·Antimicrobial Agents and Chemotherapy·J O KahnR Williams
Jan 1, 1994·Archives of Toxicology·H ZhuH G Shertzer
Apr 15, 1994·Cancer·J F KerrB V Harmon
Oct 1, 1993·Neuropathology and Applied Neurobiology·P A GarciaR O Messing
Feb 15, 1996·Biochimica Et Biophysica Acta·L M Henderson, J B Chappel
Jan 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D Dreher, A F Junod
Jan 1, 1997·Free Radical Biology & Medicine·Y J SuzukiA Sevanian
Apr 1, 1997·Cardiovascular Research·G M PieperW Siebeneich
Nov 5, 1997·The Journal of Biological Chemistry·H R Stennicke, G S Salvesen
Feb 19, 1998·Archives of Biochemistry and Biophysics·R GoldmanU Zor
Apr 30, 1998·Current Opinion in Cell Biology·T Finkel
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Feb 7, 2001·Biochemical and Biophysical Research Communications·W L ChanM S Nielsen

❮ Previous
Next ❯

Citations

Jul 25, 2009·Molecular Biology Reports·Ping WangCheng Zhang
Jan 21, 2012·Applied Biochemistry and Biotechnology·Mrinal Kumar DasVandana Mishra
Jun 23, 2018·Current Protein & Peptide Science·Chen LingChangquan Ling
May 4, 2005·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Andy SunWon-Bo Wang
Dec 22, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shilpa ChooniedassJeannick Cizeau
Jul 20, 2004·World Journal of Gastroenterology : WJG·Zhen-Hong LiJing-Xing Liu
Mar 13, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Meng LiJi-Cheng Li
Mar 1, 2005·FEBS Letters·Sriram NarayananAnjali A Karande
Aug 27, 2010·Pharmaceutical Biology·Chien-Te LiJiunn-Liang Ko
Jun 21, 2019·Toxins·Natalia Sowa-RogozińskaMonika Słomińska-Wojewódzka
May 5, 2006·Journal of Biochemistry·Xuechun XiaHuiling Nie
Mar 27, 2016·Biological Research·JinLong LiYongChen Zhang
Apr 6, 2017·Molecular and Cellular Biochemistry·Parminder K AjjiMunish Puri
Jul 9, 2004·World Journal of Gastroenterology : WJG·Chang-Ming Dou, Ji-Cheng Li
Sep 15, 2017·Journal of Toxicology and Environmental Health. Part a·Min QiuQuan-Li Liu
Apr 8, 2020·ACS Pharmacology & Translational Science·Akmal M AsrorovYongzhuo Huang
Mar 23, 2021·BMC Complementary Medicine and Therapies·Zeyao ZhuOu Sha
Apr 26, 2021·Molecular Biology Reports·Zahra Setayesh-Mehr, Mahdiye Poorsargol
Apr 5, 2005·Toxicon : Official Journal of the International Society on Toxinology·Pang-Chui ShawKam-Bo Wong
Aug 30, 2005·Biochemical and Biophysical Research Communications·Xuechun XiaHuiling Nie
Sep 25, 2007·Toxicon : Official Journal of the International Society on Toxinology·Ou ShaTzi Bun Ng
Sep 11, 2007·Biochemical and Biophysical Research Communications·Ping WangJi-Cheng Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis