Reactive oxygen species modulate Na+ -coupled neutral amino acid transporter 1 expression in piglet pulmonary arterial endothelial cells

American Journal of Physiology. Heart and Circulatory Physiology
Anna DikalovaCandice D Fike

Abstract

We have previously shown that Na+-coupled neutral amino acid transporter 1 (SNAT1) modulates nitric oxide (NO) production in pulmonary arterial endothelial cells (PAECs) from newborn piglets. Specifically, the ability to increase NO production in response to the l-arginine-NO precursor l-citrulline is dependent on SNAT1 expression. Elucidating factors that regulate SNAT1 expression in PAECs could provide new insights and therapeutic targets relevant to NO production. Our major goals were to determine if reactive oxygen species (ROS) modulate SNAT1 expression in PAECs from newborn piglets and to evaluate the role of NADPH oxidase 1 (NOX1) and uncoupled endothelial NO synthase, enzymatic sources of ROS, in hypoxia-induced increases in SNAT1 expression. Treatment with either H2O2 or xanthine plus xanthine oxidase increased SNAT1 expression in PAECs from newborn piglets cultured under normoxic conditions. Hypoxia-induced increases in SNAT1 expression were inhibited by treatments with the ROS-removing agents catalase and superoxide dismutase, NOX1 siRNA, and the NO synthase inhibitor NG-nitro-l-arginine methyl ester. Both tetrahydropbiopterin (BH4) and l-citrulline, two therapies that decrease ROS by recoupling endothelial NO syntha...Continue Reading

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Citations

Jun 5, 2019·Animals : an Open Access Journal From MDPI·Qi MouPeng-Fei Huang
Feb 20, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Anna DikalovaCandice D Fike
Jul 15, 2020·Pediatric Pulmonology·Eric B McClellanCandice D Fike

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Methods Mentioned

BETA
electron spin resonance
protein assay
X-ray

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