Reactive oxygen species play a role in P2X7 receptor-mediated IL-6 production in spinal astrocytes.

Purinergic Signalling
Frances M MunozHuijuan Hu

Abstract

Astrocytes mediate a remarkable variety of cellular functions, including gliotransmitter release. Under pathological conditions, high concentrations of the purinergic receptor agonist adenosine triphosphate (ATP) are released into the extracellular space leading to the activation of the purinergic P2X7 receptor, which in turn can initiate signaling cascades. It is well-established that reactive oxygen species (ROS) increase in macrophages and microglia following P2X7 receptor activation. However, direct evidence that activation of P2X7 receptor leads to ROS production in astrocytes is lacking to date. While it is known that P2X7R activation induces cytokine production, the mechanism involved in this process is unclear. In the present study, we demonstrated that P2X7 receptor activation induced ROS production in spinal astrocytes in a concentration-dependent manner. We also found that P2X7R-mediated ROS production is at least partially through NADPH oxidase. In addition, our ELISA data show that P2X7R-induced IL-6 release was dependent on NADPH oxidase-mediated production of ROS. Collectively, these results reveal that activation of the P2X7 receptor on spinal astrocytes increases ROS production through NADPH oxidase, subsequent...Continue Reading

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Citations

Mar 2, 2021·Frontiers in Molecular Neuroscience·Ya-Fei ZhaoPeter Illes
Nov 9, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Luísa Teixeira-SantosDora Pinho
Jul 27, 2021·Journal of Inflammation Research·Tobias EngelMariana Alves

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay

Software Mentioned

MetaFluor
ImageJ
GraphPad Prism

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