Reactive Oxygen Species (ROS) in human semen: determination of a reference range

Journal of Assisted Reproduction and Genetics
Sheryl T HomaAshok Agarwal

Abstract

High levels of reactive oxygen species (ROS) are a leading cause of male factor infertility. Measurement of ROS has been hampered by a lack of standardisation and confounding variables including choice of controls and sample selection. This study aimed to determine a reference range for ROS in human semen. Semen samples were obtained from men attending for routine semen analysis who gave informed consent for the study. Samples were assigned groups: Group 1 (N = 94) normal semen parameters, no leucocytospermia; Group 2 (N = 100) abnormal semen parameters, no leucocytospermia; Group 3 (N = 41) any semen parameters with leucocytospermia. ROS levels were assayed in fresh neat semen using a chemiluminescence assay measured in a single tube luminometer. Data are reported in relative light units (RLU)/sec/10(6) sperm ROS levels were significantly different between Groups 1, 2 and 3 (19.75 ± 8.12, 95.03 ± 33.63, 890.17 ± 310.23 RLU/sec/10(6) sperm respectively; p < 0.001). Group 3 gave the highest value confirming this group as the optimum choice for positive controls. The reference range < 24.1 RLU/sec/10(6) sperm was determined by ROC analysis that differentiates a reference population (Group 1) from a positive control group (Group 3...Continue Reading

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Citations

Mar 5, 2016·Journal of Assisted Reproduction and Genetics·Shubhadeep RoychoudhuryAshok Agarwal
Dec 21, 2017·Reproductive Medicine and Biology·Yasushi YumuraAkira Iwasaki
Oct 5, 2018·Critical Reviews in Analytical Chemistry·Y KazakovK Brainina
Nov 2, 2018·Journal of Assisted Reproduction and Genetics·Mahsa DarbandiMohammad Reza Sadeghi
Nov 28, 2019·Andrologia·Fayez A AlmabhouhHarbindar Jeet Singh
Mar 25, 2019·Human Fertility : Journal of the British Fertility Society·Sylvia YanSuks Minhas
Jul 10, 2019·Arab Journal of Urology·Sulagna DuttaAshok Agarwal
Feb 18, 2020·Biodemography and Social Biology·Ronny Westerman
Sep 22, 2018·Human Reproduction·Giacomo LazzarinoPasquale Bilotta
Dec 31, 2020·Andrologia·Anna VassiliouAideen P Killeen
Mar 10, 2021·Andrologia·Fernando LopesRosário Pinto-Leite
Apr 4, 2021·Biology·Elizabeth Torres-ArceAlbert Salas-Huetos
May 31, 2021·Andrologia·Rihab DerbelLeila Ammar-Keskes
Jul 3, 2021·International Journal of Molecular Sciences·Safeera KhanZhenquan Jia

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