PMID: 11931015Apr 5, 2002Paper

Reactive oxygen species (ROS) production by amoebocytes of Asterias rubens (Echinodermata)

Fish & Shellfish Immunology
Geoffroy CoteurPhilippe Dubois

Abstract

An adapted peroxidase, luminol-enhanced chemiluminescence method in an EDTA-free, Ca++-containing medium is described and used to characterise reactive oxygen species (ROS) production by starfish immunocytes using a standard microplate reader luminometer. ROS production was stimulated by direct interaction of immunocytes with bacteria or bacterial wall components, but not by the soluble stimulant PMA nor the lectin concanavalin A. Produced ROS detected by this method are apparently superoxide anions, hydrogen peroxide and peroxynitrite. Comparison with other chemiluminescence methods indicates that the described method is the only one to detect the stimulation of starfish immunocytes by the Gram-positive bacteria, Micrococcus luteus, a fact that questions previous reports indicating a lack of stimulation by pathogens. The adapted method provides a rapid determination of the overall ROS production, which is suitable for both disease control and immunotoxicological studies in echinoderms.

References

Jul 1, 1992·Developmental and Comparative Immunology·T ItoT Nomura
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S BeckmanB A Freeman
May 1, 1987·Journal of Invertebrate Pathology·R DikkeboomW P van der Knaap
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G Beck, G S Habicht
Jan 1, 1985·Developmental and Comparative Immunology·M NakamuraT Nomura
Mar 1, 1984·The Journal of Clinical Investigation·B M Babior
Jan 1, 1980·Developmental and Comparative Immunology·J Gebbinck
May 1, 1997·Developmental and Comparative Immunology·J TorreillesP Roch
Sep 12, 2000·Developmental and Comparative Immunology·G BeckN Truong
Jan 1, 1999·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·R S Anderson

❮ Previous
Next ❯

Citations

Apr 6, 2011·Fish & Shellfish Immunology·R P EllisS Widdicombe
Jul 22, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Fangyu WangGuangbin Liu
Jun 2, 2007·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Natarajan VidyaMunusamy Arumugam
Jan 24, 2007·Developmental and Comparative Immunology·Geoffroy CoteurHåkan Steiner
Apr 27, 2016·Developmental and Comparative Immunology·A RomeroA Figueras
May 7, 2008·Archives of Environmental Contamination and Toxicology·Guillemette Joly-TurquinMonique Guillou
Jun 1, 2020·Ecotoxicology and Environmental Safety·Hany M R Abdel-LatifMansour El-Matbouli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.