Reactivities of human sera with human herpesvirus-8-infected BCBL-1 cells and identification of HHV-8-specific proteins and glycoproteins and the encoding cDNAs

Virology
B ChandranE Goldstein

Abstract

The reactivates of human sera with uninduced and phorbol ester (TPA)-induced human herpesvirus-8 (HHV-8)-infected BCBL-1 cells were examined by immunofluorescence assay (IFA) and by radioimmunoprecipitation reactions (RIP). The seroprevalence of HHV-8 infections is low in the United States general population and only low levels of HHV-8 antibodies were detected in the seropositive sera. In contrast, high levels of antibodies against HHV-8 lytic and latent antigens were detected by IFA in the sera from HIV+ Kaposi's sarcoma (KS)-positive individuals. These sera recognized several proteins and glycoproteins from BCBL-1 cells in RIP reactions. Two types of antibody responses were detected in the sera from HIV+ KS- homosexual men. In majority of the sera with and without detectable HHV-8 DNA in the peripheral blood mononuclear cells (PBMC), significantly low levels of HHV-8 antibodies were detected by IFA. These sera recognized only a subset of HHV-8 proteins and glycoproteins in RIP reactions. In contrast, in a subgroup of sera from HIV+ KS- homosexual men, higher levels of IFA antibodies against HHV-8 lytic and latent antigens were detected. These sera also recognized several viral proteins and glycoproteins in RIP reactions. The...Continue Reading

References

Jun 1, 1989·Journal of Virology·N BalachandranC K Chang
May 4, 1995·The New England Journal of Medicine·E CesarmanD M Knowles
Mar 25, 1995·Lancet·Y Q HuangA E Friedman-Kien
Oct 1, 1996·Histopathology·I Anagnostopoulos, M Hummel
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·J J RussoP S Moore

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Citations

Nov 9, 2000·The New England Journal of Medicine·J PaukL Corey
Apr 21, 2001·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C Boshoff, R A Weiss
Nov 28, 2002·Journal of Clinical Microbiology·Francesco BroccoloMauro S Malnati
Jan 18, 2006·Journal of Virology·Christine M O'Connor, Dean H Kedes
Sep 6, 2005·Virology Journal·Daniel C Edelman
Nov 25, 2011·PLoS Pathogens·Bénédicte MachielsLaurent Gillet
Apr 13, 2004·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Corey CasperAnna Wald
Oct 12, 2000·The Journal of Infection·T F Schulz
Apr 7, 2004·Microbes and Infection·Paolo De Paoli
Dec 13, 2000·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·J C LeaoC Scully
Feb 13, 2016·Infectious Agents and Cancer·Rachel A Bender IgnacioCorey Casper
May 22, 2007·Journal of Virological Methods·Tomoe KuharaYoshinari Matsumoto
Apr 3, 2014·Journal of Medical Virology·Warren PhippsR Scott McClelland
Feb 14, 2002·The Journal of General Virology·Lesley J Coulter, Hugh W Reid
Feb 23, 2010·Virology·Young C ShinRonald C Desrosiers
Sep 24, 2002·Revista do Hospital das Clínicas·Jair Carneiro LeãoCrispian Scully
Sep 15, 2000·Sexually Transmitted Diseases·A ZagoR Keller
Oct 24, 2001·Scandinavian Journal of Infectious Diseases·M Enbom
May 4, 2004·Oncogene·Shaw M AkulaJames A McCubrey
Jun 9, 2005·Critical Reviews in Clinical Laboratory Sciences·Adina CohenRonit Sarid
Jan 15, 2003·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R TedeschiP De Paoli

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