Abstract
A total of sixteen recombinant variants of a major house dust mite allergen Der f 2, the wild-type Der f 2, six cysteine mutants, six proline mutants, and three lysine mutants, were expressed in Escherichia coli. The cells were solubilized and run on SDS-PAGE under reducing conditions. Epitopes for five mouse anti-Der f 2 monoclonal antibodies, 1B2, 7C10, 13A4, 15E11, and 18G8, to the recombinant Der f 2 variants were characterized by immunoblot analysis.
References
Nov 1, 1992·Bioscience, Biotechnology, and Biochemistry·M AkagawaH Okudaira
May 1, 1991·Agricultural and Biological Chemistry·T YuukiH Okudaira
Mar 1, 1991·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K Y ChuaW R Thomas
Nov 1, 1991·Molecular Immunology·W van 't HofR C Aalberse
Jan 1, 1991·International Archives of Allergy and Applied Immunology·T YuukiH Okudaira
Jan 1, 1990·International Archives of Allergy and Applied Immunology·K Y ChuaW R Thomas
Sep 1, 1994·The Journal of Allergy and Clinical Immunology·I G OvsyannikovaM D Chapman
Sep 1, 1994·International Archives of Allergy and Immunology·C NishiyamaH Okudaira
Jan 1, 1993·International Archives of Allergy and Immunology·C NishiyamaH Okudaira
Oct 1, 1995·Molecular Immunology·C NishiyamaH Okudaira
Mar 1, 1996·Molecular Immunology·A M Smith, M D Chapman
May 1, 1997·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A M Smith, M D Chapman
Feb 1, 1997·Molecular Immunology·T TakaiH Okudaira
Aug 1, 1997·Nature Biotechnology·T TakaiY Okumura
Feb 7, 1998·The Journal of Biological Chemistry·S IchikawaF Inagaki
Apr 16, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G A HakkaartR Van Ree
Sep 16, 1998·Biochemistry·G A MuellerG S Rule
Jun 16, 1999·Molecular Immunology·C NishiyamaY Okumura
Mar 4, 2000·Molecular Immunology·T TakaiY Okumura
Oct 29, 2000·European Journal of Biochemistry·T TakaiY Okumura
Nov 9, 2000·FEBS Letters·T TakaiY Okumura
Citations
Aug 23, 2001·Bioscience, Biotechnology, and Biochemistry·T TakaiY Okumura
Mar 24, 2005·International Archives of Allergy and Immunology·Toshiro TakaiHideoki Ogawa
Feb 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Piia KarisolaHarri Alenius