Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes

Veterinary Immunology and Immunopathology
H GalkowskaU Wojewodzka

Abstract

A panel of anti-human antibodies was used in order to investigate their cross-reactivity with canine leukocytes. The labeling was carried out at the microscopic level by immunocytochemical staining. Of 50 antibodies 22 cross-reacted with canine leukocytes from afferent lymph and peripheral blood. All leukocytes reacted with MHM23 (CD18). Two anti-HLA DR antibodies, DK22 and L243, reacted mostly with veiled cells and with PHA-stimulated lymphocytes, whereas TAL1B5 was cross-reactive with all canine lymphocytes. The activation markers CD25, Ki-67, PCNA were identified on PHA-stimulated lymphocytes with ACT1, Ki-67 and PC10 clone produced antibodies. Canine eosinophils reacted with MHM6 (CD23) antibody. A large number of antibodies reacted with canine lymph veiled cells. Canine granulocytes and a subset of lymphocytes were stained with the anti-CD15 antibody only after treatment of cytospins with neuraminidase.

Citations

Oct 19, 2006·Journal of Molecular Histology·Eberhard BuseFriedhelm Vogel
Aug 21, 2004·Hybridoma and Hybridomics·Paulo Henrique Palis AguiarLain Pontes-de-Carvalho
May 29, 2004·Hybridoma and Hybridomics·Paulo Henrique Palis AguiarLain Pontes-de-Carvalho
Jan 12, 2010·Veterinary Immunology and Immunopathology·V Kraig AbramsRichard A Nash
Aug 15, 2006·Veterinary Immunology and Immunopathology·Viskam WijewardanaToshio Inaba
Apr 20, 2016·Veterinary Immunology and Immunopathology·Noriyuki MizutaniHajime Tsujimoto
Sep 6, 2008·The Journal of Veterinary Medical Science·Sanae ShibataTsuneo Fukata
Mar 12, 2015·Veterinary Immunology and Immunopathology·Ashley N Hartley, Rick L Tarleton
Jun 4, 2018·Developmental and Comparative Immunology·Sita S WithersRobert B Rebhun

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