Reactivity of convalescent-phase hemolytic-uremic syndrome patient sera with the megaplasmid-encoded TagA protein of Shiga toxigenic Escherichia coli O157.

Journal of Clinical Microbiology
Adrienne W Paton, James C Paton

Abstract

A cosmid library of Shiga toxigenic Escherichia coli (STEC) O157:H7 strain EDL933 DNA was screened for clones capable of reacting with convalescent-phase serum from a patient with hemolytic-uremic syndrome (HUS), in an attempt to identify candidate virulence genes. One of the immunoreactive clones contained a portion of the large plasmid pO157, and the immunoreactive gene product was identified as TagA. The function of this 898-amino-acid protein is unknown, but it exhibits 42% amino acid sequence identity and 63% similarity to a 312-amino-acid region of a ToxR-regulated lipoprotein of Vibrio cholerae. Antibodies to E. coli O157 TagA were detected in sera from other HUS patients with O157 STEC infection but not in those from patients whose illnesses were caused by other STEC types or in healthy controls. These data demonstrate that TagA is expressed in vivo and provide circumstantial evidence for a role in the pathogenesis of the disease. The tagA gene is present only in STEC strains belonging to serogroup O157, and so antibodies to TagA are a potentially useful serological marker for infections due to such strains.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C ParsotJ J Mekalanos
Jan 1, 1989·Clinical Microbiology Reviews·M A Karmali
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Mar 1, 1997·Trends in Microbiology·M S DonnenbergB B Finlay
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·D K KaraolisP R Reeves
Jun 17, 1998·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·K MakinoH Shinagawa
Jul 17, 1998·Clinical Microbiology Reviews·J C Paton, A W Paton
Feb 24, 2001·Nature·N T PernaF R Blattner
Mar 22, 2001·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T HayashiH Shinagawa

❮ Previous
Next ❯

Citations

Apr 21, 2004·The Journal of Experimental Medicine·Wyndham W LathemRodney A Welch
Jun 22, 2006·Journal of Bacteriology·Thomas E GrysRodney A Welch
Feb 28, 2009·PLoS Pathogens·Rose L SzabadyRodney A Welch
Mar 6, 2013·Journal of Structural Biology·Konstantin V Korotkov, Wim G J Hol
Mar 27, 2004·FEMS Microbiology Letters·H S KumarShinji Yamasaki
Jun 23, 2009·International Journal of Food Microbiology·Camilla SekseHannah Joan Jørgensen
Jan 23, 2009·International Journal of Medical Microbiology : IJMM·Rita PragerHelmut Tschäpe
Jan 7, 2016·Medicine·Morgane CommereucAlexandre Hertig

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.