PMID: 7537751Feb 1, 1995Paper

Reactivity of five anti-HIV-1 subtype O specimens with six different anti-HIV screening ELISAs and three immunoblots

Journal of Virological Methods
L GürtlerS Knapp

Abstract

Five anti-subtype O specimens were tested by anti-HIV-1/2 screening and confirmatory assays. They can be divided into three specimens, reactive with all ELISAs, independent of the nature of the antigen (recombinant proteins or peptides) and test configuration (indirect ELISA or double antigen/sandwich ELISA). One specimen was not detected by one peptide based ELISA. One specimen was only recognized by two ELISAs and should be considered as a marker sample for the weakness of currently used ELISAs with anti-subtype O. Three different immunoblot assays available commercially detected two of the specimens with a major binding of gp160 and other viral bands, especially the integrase and reverse transcriptase. Another two specimens lacked reactivity with glycoproteins almost completely, but showed some staining with the enzymes of HIV, and would most probably be interpreted as indeterminate. The fifth specimen, which was also missed by most of the ELISAs, had very faint staining of the gp160 and a very weak staining of p24, and would most probably be interpreted as negative. Adaption of currently available tests to anti-subtype O is needed for the future reliability of anti-HIV diagnostic reagents.

References

Jan 1, 1987·Journal of Virological Methods·L G GürtlerF Deinhardt
Jun 4, 1994·Lancet·I Loussert-AjakaF Simon
Mar 1, 1993·AIDS Research and Human Retroviruses·S BruistenH Huisman

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Citations

Jul 1, 1996·Journal of Virological Methods·J van BinsbergenL G Gürtler
Apr 28, 1999·Bioorganic & Medicinal Chemistry·A J KeselW Oberthür
Sep 1, 1997·AIDS Research and Human Retroviruses·J HackettS G Devare
Jul 1, 1997·AIDS Research and Human Retroviruses·C A BrennanS G Devare
May 22, 2001·AIDS Patient Care and STDs·K Gould
Jul 20, 1996·Lancet·L Gürtler
Nov 1, 1995·Infection·H HamplL Gürtler
Mar 4, 2000·Pediatric Clinics of North America·K Nielsen, Y J Bryson
Mar 12, 1998·Infectious Disease Clinics of North America·H W Jaffe, G Schochetman
Apr 20, 2006·Journal of Medical Virology·Catherine A BrennanJohn Hackett

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