PMID: 2866791Aug 27, 1985Paper

Reactivity of Glu-22(beta) of hemoglobin S for amidation with glucosamine

Biochemistry
A S Acharya, R Seetharam

Abstract

X-ray diffraction analysis of deoxyhemoglobin S crystals has implicated that a number of carboxyl groups of the protein are present at or near the intermolecular contact regions. The reactivity of these or other carboxyl groups of hemoglobin S for the amidation with an amino sugar, i.e., glucosamine, and the influence of amidation on the oxygen affinity and polymerization have been investigated. Reaction of oxyhemoglobin S at pH 6.0 and 23 degrees C with 20 mM 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide (EDC) and 100 mM [3H]glucosamine for 1 h resulted in an incorporation of nearly two residues of glucosamine per tetramer. The amidation was very specific for the carboxyl groups of globin; the glucosamine was not incorporated into the heme carboxyls. Derivatization of hemoglobin S by glucosamine increased the O2 affinity of the protein but had no influence on either the Hill coefficient or the Bohr effect. Amidation by glucosamine also increased the solubility of deoxyhemoglobin S by about 55%. Tryptic peptide mapping of the modified hemoglobin S indicated that the peptides beta-T3 and beta-T5 contained the glucosamine incorporated into the protein. Sequence analysis of glucosamine-modified beta-T3 and beta-T5 demonstrated t...Continue Reading

References

Aug 15, 1978·Analytical Biochemistry·R E BeneschR Benesch
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R L NagelK Matsutomo
Oct 5, 1978·The New England Journal of Medicine·J Dean, A N Schechter
Mar 1, 1978·Analytical Biochemistry·D G KlapperJ D Capra
Oct 19, 1978·The New England Journal of Medicine·J Dean, A N Schechter
Feb 1, 1977·Analytical Biochemistry·C L ZimmermanJ J Pisano
Oct 15, 1975·Journal of Molecular Biology·B C WishnerW E Love
Jan 1, 1973·British Journal of Haematology·S Rahbar
Apr 1, 1967·Chemical Reviews·F Kurzer, K Douraghi-Zadeh
Jun 1, 1966·The Journal of Clinical Investigation·S CharacheJ B Clegg
Feb 21, 1967·Biochemical and Biophysical Research Communications·B H BowmanR Hite
Jun 1, 1984·International Journal of Peptide and Protein Research·R D Waniska, J E Kinsella
Apr 27, 1984·Biochimica Et Biophysica Acta·M D RhodaJ Rosa
Jan 1, 1981·Annual Review of Biochemistry·F Wold
Jul 1, 1983·International Journal of Peptide and Protein Research·A S AcharyaJ M Manning
Aug 25, 1981·Journal of Molecular Biology·S J Edelstein
Jan 1, 1964·Advances in Protein Chemistry·A ROSSIFANELLIA CAPUTO
Jan 1, 1972·Methods in Enzymology·K L Carraway, D E Koshland

❮ Previous
Next ❯

Citations

Jan 1, 1992·Biomaterials, Artificial Cells, and Immobilization Biotechnology : Official Journal of the International Society for Artificial Cells and Immobilization Biotechnology·M BrouwerJ Bonaventura
Aug 18, 1995·The Journal of Biological Chemistry·M J RaoA S Acharya
Jul 19, 2015·The Journal of Biological Chemistry·Ming F TamChien Ho

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.