Reactivity of smooth-muscle antibodies, surface ultrastructure, and mobility in cells of human hematopoietic cell lines.

Journal of the National Cancer Institute
A FagraeusR Norberg

Abstract

Seventeen human hematopoietic cell lines were tested by indirect immunofluorescence (IF) for reactivity with human serum containing smooth-muscle antibodies (SMA). The correlation of the IF pattern to the cell surface ultrastructure was revealed by scanning electron microscopy (SEM). Lymphoma cells, viewed by SEM, had short villi over the entire cell surface, but, by IF, showed a type of membrane fluorescence. Cells of lymphoblastoid lines had thin, long surface villi, sometimes asymmetric but most often distributed over the whole cell surface. Myeloma and leukemia cells, which had few membrane villi but a surface covered by "blebs" as revealed by SEM, demonstrated, by IF, only a few stub-like projections extending from the surface. Time-lapse cinematography revealed that the intensity and pattern of SMA staining were also correlated to the degree of motility. Indirect IF with human SMA-positive serum might be used in the classification of cell lines derived from human hematopoietic tissue.

Citations

Apr 15, 1979·International Journal of Cancer. Journal International Du Cancer·W P van BeekP Emmelot
Jan 1, 1976·Scandinavian Journal of Immunology·K NilssonH Mellstedt
Jan 15, 1976·International Journal of Cancer. Journal International Du Cancer·L ZechG Klein
Mar 15, 1977·International Journal of Cancer. Journal International Du Cancer·K NilssonG Klein

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