PMID: 2511267Dec 1, 1989Paper

Reactivity of V beta 17a+ CD8+ T cell hybrids. Analysis using a new CD8+ T cell fusion partner

The Journal of Experimental Medicine
H G BurgertJ Kappler

Abstract

Tolerance to IE molecules leads to deletion of V beta 17a-bearing T cells. Both, the CD4+ as well as the CD8+ T cell subsets are affected. A large percentage of CD4+ V beta 17a+ T cell hybrids recognize IE molecules. We now have investigated the reactivity for IE antigens of CD8+ V beta 17a+ T cell hybrids. Using a transfection approach, we have introduced the murine CD8 molecule into different V beta 17a+ T cell hybrids. Furthermore, the CD8 cDNA was transfected into the BW5147 alpha-beta- fusion partner. This allowed us to generate a large number of V beta 17a+ T cell hybrids by fusion with the appropriate T cells. Only 6% of T cell hybrids were stimulated to produce IL-2 upon incubation with IE+ cells. However, in those, the CD8 molecule seemed not to contribute to the IE reactivity of the hybrid, since mAbs against the CD8 molecule failed to inhibit their reactivity. This low percentage of V beta 17a+ CD8+ IE-reactive T cell hybrids contrasts with the strong reduction of CD8+ V beta 17a+ T cells in IE+ mice, strongly suggesting that elimination of such cells in the thymus occurs when they are coexpressing CD4 and CD8. This view was confirmed by the occasional expression of CD4 in some hybrids in which case IE reactivity was...Continue Reading

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Citations

Oct 29, 2003·Journal of Immunological Methods·David H CanadayW Henry Boom
May 18, 2004·Nature Immunology·Antoine PerchelletJoan Goverman
Dec 12, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·N V SerbinaJ L Flynn
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Mar 1, 1992·The Journal of Experimental Medicine·N A Hosken, M J Bevan
Jul 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·E M Hiltbold, H K Ziegler
Apr 25, 2008·Current Protocols in Immunology·A M Kruisbeek
Apr 25, 2008·Current Protocols in Immunology·C V Harding
Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rakesh BagaiPeter S Heeger
Feb 10, 2010·Current Protocols in Immunology·Clifford V Harding, Lakshmi Ramachandra
Feb 10, 2010·Current Protocols in Immunology·Clifford V HardingLakshmi Ramachandra
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·P J Chefalo, C V Harding
May 19, 2020·Archivum Immunologiae Et Therapiae Experimentalis·Janice WhiteWilli K Born

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