Reading of the non-template DNA by transcription elongation factors

Molecular Microbiology
Vladimir Svetlov, Evgeny Nudler

Abstract

Unlike transcription initiation and termination, which have easily discernable signals, such as promoters and terminators, elongation is regulated through a dynamic network involving RNA/DNA pause signals and states-rather than sequence-specific protein interactions. A report by Nedialkov et al. () provides experimental evidence for sequence-specific recruitment of elongation factor RfaH to transcribing RNA polymerase (RNAP) and outlines the mechanism of gene expression regulation by restraint ('locking') of the DNA non-template strand. According to this model, the elongation complex pauses at the so called 'operon polarity sequence' (found in some long bacterial operons coding for virulence genes), when the usually flexible non-template DNA strand adopts a distinct hairpin-loop conformation on the surface of transcribing RNAP. Sequence-specific binding of RfaH to this DNA segment facilitates conversion of RfaH from its inactive closed to its active open conformation. The interaction network formed between RfaH, non-template DNA and RNAP locks DNA in a conformation that renders RNAP resistant to pausing and termination. The effects of such locking on elongation can be mimicked by restraint of the non-template strand due to its ...Continue Reading

References

Jan 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anastasiya SevostyanovaIrina Artsimovitch
Feb 6, 2010·Molecular Microbiology·Georgiy A BelogurovIrina Artsimovitch
Mar 2, 2011·The Journal of Biological Chemistry·Milija JovanovicMartin Buck
Mar 10, 2011·The EMBO Journal·Fuensanta W Martinez-RucoboPatrick Cramer
Jul 23, 2011·Molecular Cell·Anastasia SevostyanovaIrina Artsimovitch
Feb 5, 2013·Cell·Albert WeixlbaumerSeth A Darst
Jun 19, 2013·Molecular Cell·Dhananjaya NayakRobert Landick
Jan 10, 2014·Nature·Vitaly EpshteinEvgeny Nudler
Feb 3, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Monali NandyMazumdar, Irina Artsimovitch
Jul 2, 2015·Annual Review of Microbiology·Georgiy A Belogurov, Irina Artsimovitch
Jan 30, 2016·Trends in Biochemical Sciences·Jinwei Zhang, Robert Landick
May 21, 2016·Science·Venu KamarthapuEvgeny Nudler
Sep 13, 2016·Molecular Cell·Diego DuchiAchillefs N Kapanidis
Dec 3, 2016·Nucleic Acids Research·Bin Liu, Thomas A Steitz
Dec 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Monali NandyMazumdarIrina Artsimovitch
Apr 30, 2017·Nature Microbiology·Nelly SaidMarkus C Wahl
Jun 21, 2017·DNA Repair·Bibhusita Pani, Evgeny Nudler
Mar 3, 2018·Molecular Cell·Xieyang GuoAlbert Weixlbaumer
May 15, 2018·Molecular Microbiology·Yuri NedialkovIrina Artsimovitch

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Citations

Jun 14, 2021·Journal of Molecular Biology·Amr ElghondaklyWade C Winkler

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