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Reagent and laboratory contamination can critically impact sequence-based microbiome analyses

BMC Biology

Nov 12, 2014

Susannah J. SalterAlan W Walker

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Abstract

The study of microbial communities has been revolutionised in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. One potential confounder of these sequence-based approaches is the presence of contaminat...read more

Mentioned in this Paper

RNA, Ribosomal, 16S
Salmonella
Sequence Determinations, DNA
DNA Contamination
Microbiota (plant)
Microbiome
Indicators
Metagenomics
Nested Polymerase Chain Reaction
Microbial
264
11
9
1742
1
1
1
Paper Details
References
  • References66
  • Citations297
12345...
  • References66
  • Citations297
12345...

Reagent and laboratory contamination can critically impact sequence-based microbiome analyses

BMC Biology

Nov 12, 2014

Susannah J. SalterAlan W Walker

PMID: 25387460

DOI: 10.1186/s12915-014-0087-z

Abstract

The study of microbial communities has been revolutionised in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. One potential confounder of these sequence-based approaches is the presence of contaminat...read more

Mentioned in this Paper

RNA, Ribosomal, 16S
Salmonella
Sequence Determinations, DNA
DNA Contamination
Microbiota (plant)
264
11
9
1742
1
1
1
Paper Details
References
  • References66
  • Citations297
12345...
  • References66
  • Citations297
12345...

Get paper from

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