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Reagent contamination can critically impact sequence-based microbiome analyses

bioRxiv

Jul 16, 2014

Susannah J. SalterAlan W Walker

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Abstract

The study of microbial communities has been revolutionised in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. One potential confounder of these sequence-based approaches is the presence of contaminat...read more

Mentioned in this Paper

Gene Delivery Systems
Polymerase Chain Reaction
Study
Shotgun protein, Drosophila
Extraction
Genome Sequencing
Bacterial 16S RNA
Microbiota (plant)
Microbiome
Base Sequence
174
1
1
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  • Reagent contamination can critically impact sequence-based microbiome analyses

    bioRxiv

    Jul 16, 2014

    Susannah J. SalterAlan W Walker

    PMID: 990007187

    DOI: 10.1101/007187

    Abstract

    The study of microbial communities has been revolutionised in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. One potential confounder of these sequence-based approaches is the presence of contaminat...read more

    Mentioned in this Paper

    Gene Delivery Systems
    Polymerase Chain Reaction
    Study
    Shotgun protein, Drosophila
    Extraction
    174
    1
    1

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