Real-Time and Conventional PCR Tools for Detection and Discrimination of Calonectria pseudonaviculata and C. henricotiae Causing Boxwood Blight

Plant Disease
Yonghong Guo, Margaret Pooler

Abstract

Calonectria pseudonaviculata and C. henricotiae are the causal agents of boxwood blight, a devastating disease of boxwood that has caused significant economic impact on the nursery and landscape industries in the U.S. and in Europe. The two species are genetically distinct and are found in different geographic areas but are difficult to distinguish based on morphology and pathogenicity. Fast, accurate, and inexpensive methods to detect and differentiate these species is critical in stopping the spread of the disease. We designed primer pairs based on available sequences of four conserved regions-calmodulin, histone H3, internal transcribed spacer, and β-tubulin-and tested their ability to differentiate the two Calonectria species. Here we report three primer pairs derived from sequence differences in the histone H3 region that can be used to specifically detect C. pseudonaviculata, C. henricotiae, or both species. Specificity of these primers was tested against nine isolates of C. pseudonaviculata, three isolates of C. henricotiae, 13 other Calonectria species, and five isolates from related genera using conventional and real-time PCR. These are the first primers available that can be used with either a multiplexed conventional...Continue Reading

References

Jul 11, 1995·Nucleic Acids Research·J J SteinerL F Elliott
Sep 2, 2010·Studies in Mycology·L LombardM J Wingfield
May 21, 2016·Scientific Reports·Martha Malapi-WightJo Anne Crouch
Jul 14, 2017·Biomolecular Detection and Quantification·Amin ForootanMikael Kubista
Nov 22, 2017·PLoS Biology·Yiping ZouJose Ramon Botella
Apr 4, 2018·Applied Microbiology and Biotechnology·Nicholas LeBlancJo Anne Crouch
Jul 10, 2019·Annual Review of Phytopathology·Margery L Daughtrey

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