Real-time and high-throughput Raman signal extraction and processing in CARS hyperspectral imaging

Optics Express
Charles H CampYoung Jong Lee

Abstract

We present a new collection of processing techniques, collectively "factorized Kramers-Kronig and error correction" (fKK-EC), for (a) Raman signal extraction, (b) denoising, and (c) phase- and scale-error correction in coherent anti-Stokes Raman scattering (CARS) hyperspectral imaging and spectroscopy. These new methods are orders-of-magnitude faster than conventional methods and are capable of real-time performance, owing to the unique core concept: performing all processing on a small basis vector set and using matrix/vector multiplication afterwards for direct and fast transformation of the entire dataset. Experimentally, we demonstrate that a 703026 spectra image of chicken cartilage can be processed in 70 s (≈ 0.1 ms / spectrum), which is ≈ 70 times faster than with the conventional workflow (≈7.0 ms / spectrum). Additionally, we discuss how this method may be used for machine learning (ML) by re-using the transformed basis vector sets with new data. Using this ML paradigm, the same tissue image was processed (post-training) in ≈ 33 s, which is a speed-up of ≈ 150 times when compared with the conventional workflow.

References

Feb 1, 1975·Biopolymers·B G Frushour, J L Koenig
Oct 23, 2003·Analytical Chemistry·Paul H C Eilers
Jan 31, 2006·Optics Letters·Eric O PotmaX Sunney Xie
Sep 5, 2007·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Michiel Müller, Andreas Zumbusch
Dec 1, 2007·Optics Letters·J X ChengX S Xie
Sep 1, 1975·Applied Optics·T S Huang, P M Narendra
Nov 26, 2010·Cancer Research·Praveen D ChowdaryStephen A Boppart
Jan 17, 2012·Physical Review Letters·Erik T GarbacikHerman L Offerhaus
Dec 18, 2012·Journal of Biophotonics·Adrian F PegoraroAlbert Stolow
May 31, 2014·Biomedical Optics Express·Claudia Di NapoliPaola Borri
Oct 15, 2014·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Raja Giryes, Michael Elad
Jan 27, 2015·Nature Photonics·Charles H CampMarcus T Cicerone
Apr 1, 2016·Journal of Raman Spectroscopy : JRS·Charles H CampMarcus T Cicerone

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