Real-time crystallographic studies of the adenine riboswitch using an X-ray free-electron laser

The FEBS Journal
J R StagnoYun-Xing Wang

Abstract

Structures of the four reaction states of the adenine riboswitch aptamer domain, including a transient intermediate state were solved by serial femtosecond crystallography. The structures not only demonstrate the use of X-ray free-electron lasers for RNA crystallography but have also proven that transient states can be determined in real time by mix-and-inject crystallography. These results illustrate the structural basis for the ligand-induced conformational changes associated with the molecular 'switch'.

References

Sep 27, 2002·Chemistry & Biology·Ali NahviRonald R Breaker
Oct 3, 2003·Chembiochem : a European Journal of Chemical Biology·Wade C Winkler, Ronald R Breaker
May 3, 2006·Journal of Molecular Biology·Sunny D GilbertRobert T Batey
Feb 3, 2007·Methods in Molecular Biology·Barbara L Golden
Apr 19, 2007·Chembiochem : a European Journal of Chemical Biology·Renate RiederRonald Micura
Jan 5, 2008·Science·William J GreenleafSteven M Block
Jul 3, 2008·The Journal of Biological Chemistry·Andrew D GarstRobert T Batey
Jul 20, 2010·Structure·Colby D StoddardRobert T Batey
May 20, 2011·The Journal of Biological Chemistry·Jermaine L JenkinsJoseph E Wedekind
Jun 10, 2011·Nucleic Acids Research·Krishna NeupaneMichael T Woodside
Aug 2, 2012·Quarterly Reviews of Biophysics·Robert T Batey
Feb 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Andrea HallerRonald Micura
Apr 29, 2014·Biochimica Et Biophysica Acta·Jinwei ZhangAdrian R Ferré-D'Amaré
Sep 5, 2014·Structure·Jinwei Zhang, Adrian R Ferré-D'Amaré

❮ Previous
Next ❯

Citations

Jul 18, 2018·Physical Chemistry Chemical Physics : PCCP·Adam K SieradzanAdam Liwo
Aug 10, 2019·Acta Crystallographica. Section F, Structural Biology Communications·Robert J TrachmanAdrian R Ferré-D'Amaré
Oct 17, 2019·The FEBS Journal·Feng-Zhu ZhaoDa-Chuan Yin
May 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Michal M MatyjasikRobert T Batey
Nov 10, 2017·Essays in Biochemistry·Robert K Y ChengMichael Hennig
Jul 3, 2019·Cold Spring Harbor Perspectives in Biology·Kevin P LarsenJoseph D Puglisi
Feb 12, 2021·Journal of Structural Biology·Haley M WiltJason R Stagno
Mar 21, 2021·Nature Communications·Saminathan RamakrishnanYun-Xing Wang
Jul 24, 2021·Nature Communications·Agata ButrynAllen M Orville

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.