Real-time detection of GABA-induced synaptic glutamate release in cultured rat cortex

Neuroreport
K Torimitsu, O Niwa

Abstract

Glutamate is an important neurotransmitter in synaptic transmission. There are no methods, however, for continuous measurement of glutamate concentration at high temporal and spatial resolutions. We have developed a novel electrochemical detection method for the on-line measurement of glutamate release with nanomolar resolution in real time. Using this method, GABA was found to have a modulatory action on the synaptic glutamate release in cultured rat cortical cells. This synaptic modulation largely depends on the GABAA receptor and could be a key not only in neural development, but also in signal transduction in the brain. Our detection method is ideal for investigating such synaptic glutamate responses because of its higher sensitivity and real-time measurement capability.

References

Sep 1, 1993·Neuron·D AttwellM Szatkowski

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Citations

May 4, 2001·Neuroscience Letters·N KasaiK Torimitsu
Jun 6, 2003·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Keiichi TorimitsuNahoko Kasai
Dec 28, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nahoko KasaiKeiichi Torimitsu
Sep 27, 2000·The Journal of General Physiology·D S KohB Hille
Feb 13, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Natsumi SomaMasao Sugawara

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