Real-time fluorescence assays of alkaline phosphatase and ATP sulfurylase activities based on a novel PPi fluorescent probe

Talanta
Xiaobo WangZhilin Wang

Abstract

An anthracene-armed tetraaza macrocyclic fluorescent probe 3-(9-anthrylmethyl)-3,6,9,15-tetraazabicyclo[9.3.1]pentadeca-1(15),11,13-triene(l) for detecting Zn(2+) in aqueous medium was synthesized. L-Zn(2+) complex, showed selectivity toward pyrophosphate ion (PPi) by quenching the fluorescence in aqueous HEPES buffer (pH 7.4). Furthermore, L-Zn(2+) was also used to set up a real-time fluorescence assay for monitoring enzyme activities of alkaline phosphatase (ALP) and adenosine triphosphate sulfurylase (ATPS). In the presence of ALP inhibitor Na3VO4 and ATPS inhibitor chlorate, two enzymes activities decreased obviously, respectively.

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Citations

Aug 5, 2015·Luminescence : the Journal of Biological and Chemical Luminescence·Chunlei FanHaiping Qi
Jul 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Álvaro Martínez-CamarenaEnrique García-España
Aug 20, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yifang GaoChuan Dong
Mar 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Minhui HeXueji Zhang
Dec 7, 2018·Chemical Reviews·Teresa L MakoMindy Levine

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