Real-time fluorescence loop-mediated isothermal amplification assay for rapid and sensitive detection of Streptococcus gallolyticus subsp. gallolyticus associated with colorectal cancer

Analytical and Bioanalytical Chemistry
Qiuyuan LinJilie Kong

Abstract

The increasing threat of Streptococcus gallolyticus subsp. gallolyticus (SGG) infections has gained considerable attention for its strong association with colorectal cancer (CRC). Herein, we proposed real-time fluorescence loop-mediated isothermal amplification (LAMP) as a novel, simple, rapid, and highly sensitive assay for identifying SGG for the first time. This assay was capable of detecting SGG with initial DNA concentrations ranging from 102 to 108 copies per microliter, under isothermal conditions within 30 min via real-time fluorescence monitoring. Our method was tested for specific identification of SGG strains without cross-reaction with other Streptococcus gallolyticus subspecies and Escherichia coli. The developed LAMP shows a superior performance with shorter time and higher sensitivity compared with conventional polymerase chain reaction (PCR). Significantly, this proposed approach was successfully applied for detecting SGG in clinical urine samples, which is non-invasive diagnosis, showing excellent accuracy and reliability to discriminate healthy controls and CRC patients. For comparison, these samples were also tested against PCR assay. These results yielded an analytical sensitivity of 100% and a specificity o...Continue Reading

References

Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Dec 22, 2010·Chemical Communications : Chem Comm·Cuiping LiJingli Yan
Apr 1, 2011·The Journal of Infectious Diseases·Annemarie BoleijHarold Tjalsma
Oct 1, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Annemarie BoleijHarold Tjalsma
Oct 21, 2011·Cancer Prevention Research·Annemarie BoleijHarold Tjalsma
Feb 3, 2012·Biological Reviews of the Cambridge Philosophical Society·Annemarie Boleij, Harold Tjalsma
May 9, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Juan Corredoira-SánchezJose Varela
Mar 19, 2014·Cell Host & Microbe·Cynthia L Sears, Wendy S Garrett
Jun 2, 2014·Analytical and Bioanalytical Chemistry·Celine ZahradnikKurt Brunner
Sep 10, 2014·Nature Reviews. Microbiology·Petra LouisHarry J Flint
Sep 23, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Christoph JansMarc J A Stevens
Jan 6, 2015·The Journal of Microbiology·Tsugunori NotomiHidetoshi Kanda
Nov 6, 2015·International Journal of Cancer. Journal International Du Cancer·Julia ButtMichael Pawlita
Nov 10, 2015·Chemical Reviews·Yongxi ZhaoChunhai Fan
Dec 19, 2016·Angewandte Chemie·Yan DuAndrew D Ellington
May 10, 2017·International Journal of Cancer. Journal International Du Cancer·Julia ButtMichael Pawlita
Oct 13, 2017·Journal of Medical Microbiology·Rujirat HatrongjitAnusak Kerdsin
Jan 18, 2018·Nature Communications·A GanguliR Bashir
Jan 30, 2018·International Journal of Cancer. Journal International Du Cancer·Julia ButtDavid J Hughes
Apr 19, 2018·Frontiers in Microbiology·Ewa Pasquereau-KotulaShaynoor Dramsi
Aug 7, 2018·Biosensors & Bioelectronics·Umer FarooqShenqi Wang

❮ Previous
Next ❯

Citations

Mar 23, 2021·Journal of Medical Microbiology·Paulina GlajznerMagdalena Szemraj

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.