Real-time imaging of TRAIL-induced apoptosis of glioma tumors in vivo

Khalid ShahRalph Weissleder


Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been shown to induce apoptosis in neoplastic cells. While many previous studies have been performed in cell culture, the delivery and efficiency of TRAIL variants in vivo is less well established. Using dual substrate/reporter bioluminescence imaging (Fluc: firefly luciferase-luciferin and Rluc: Renilla luciferase-coelenterazine), we tested the efficacy of TRAIL using replication-deficient herpes simplex virus (HSV) type 1 amplicon vectors in gliomas. The cDNA for complete TRAIL and the extracellular domain of TRAIL (aa 114-281) were cloned into HSV amplicons and packaged into helper virus-free vectors. Both forms of TRAIL induced similar degrees of apoptosis in human glioma cells (Gli36) in culture within 24 h of infection with TRAIL amplicon vectors. Growth of tumors stably transfected with Fluc (Gli36fluc+) was readily monitored in vivo by bioluminescence imaging following luciferin administration. HSV amplicon vectors bearing the genes for TRAIL and Rluc injected directly into Gli36fluc(+)-expressing subcutaneous gliomas revealed peak Rluc activity 36 h after intratumoral injection as determined by coelenterazine injection followed by imaging. TRAIL-treated...Continue Reading


Jan 1, 1995·Journal of Neuropathology and Experimental Neurology·T KashimaA T Campagnoni
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D L Vaux
Dec 1, 1995·Current Opinion in Cell Biology·G I EvanE Harrington
May 31, 1996·The Journal of Biological Chemistry·R M PittiA Ashkenazi
Jun 1, 1996·Current Biology : CB·S A MarstersA Ashkenazi
Feb 7, 1997·Cell·S Nagata
Feb 14, 1998·Nature Medicine·P R ContagC H Contag
Jun 5, 1998·FEBS Letters·J RiegerM Weller
Aug 28, 1998·Science·A Ashkenazi, V M Dixit
Aug 28, 1998·Science·G Evan, T Littlewood
Apr 21, 1999·Current Opinion in Cell Biology·A Ashkenazi, V M Dixit
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·A M ChinnaiyanA Rehemtulla
May 10, 2000·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·K HaradaH Ito
Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·C H ContagR S Negrin
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T S GriffithT L Ratliff
Mar 3, 2001·Neoplasia : an International Journal for Oncology Research·A RehemtullaB D Ross
Apr 3, 2001·Nature Medicine·D LawrenceA Ashkenazi
Apr 26, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·Y SaekiE A Chiocca
Aug 11, 2001·Cancer Gene Therapy·K J PulkkanenS Yla-Herttuala
Sep 8, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·T S Griffith, E L Broghammer
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·S Bhaumik, S S Gambhir
Mar 21, 2002·Nature Reviews. Cancer·Ralph Weissleder
Jul 16, 2002·Annual Review of Biomedical Engineering·Christopher H Contag, Michael H Bachmann
Sep 11, 2002·Apoptosis : an International Journal on Programmed Cell Death·H WajantP Scheurich
Sep 5, 2002·Nature Reviews. Cancer·Sanjiv Sam Gambhir
Mar 26, 2003·Cell Death and Differentiation·H N LeBlanc, A Ashkenazi
Apr 18, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Giulia Fulci, Ennio Antonio Chiocca

❮ Previous
Next ❯


Dec 29, 2004·Annals of Neurology·Khalid ShahXandra O Breakefield
Jan 11, 2005·The Journal of Pathology·Scott K Lyons
Feb 28, 2006·European Journal of Nuclear Medicine and Molecular Imaging·Yusuke InoueKuni Ohtomo
Dec 7, 2007·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·C BelmarP Martín-Duque
Dec 8, 2007·Neuroscience Bulletin·Feng Xu, Jian-Hong Zhu
Mar 29, 2008·Gene Therapy·K S AboodyM K Danks
Aug 21, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Casey A MaguireMiguel Sena-Esteves
Aug 30, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kaoru TamuraKhalid Shah
Jul 10, 2007·Nature Methods·Andreas Markus LoeningSanjiv Sam Gambhir
Sep 25, 2007·Nature Reviews. Cancer·Erik Sahai
Jul 2, 2008·Nature Reviews. Drug Discovery·Jürgen K WillmannSanjiv S Gambhir
Oct 19, 2005·Gene Therapy·Alberto L Epstein
Mar 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Laura S SasportasKhalid Shah
Nov 13, 2008·Molecular Cancer Therapeutics·Shawn HingtgenKhalid Shah
Jun 15, 2007·PloS One·Alexandra WinkelerAndreas H Jacobs
Apr 25, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Khalid ShahRalph Weissleder
May 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Khalid Shah, Ralph Weissleder
Feb 16, 2005·Developmental Neuroscience·Khalid ShahXandra O Breakefield
Dec 29, 2007·Immunological Investigations·Nadir AskenasyDaniel L Farkas
Jun 4, 2011·Expert Opinion on Biological Therapy·Peggy Richard-FiardoBéatrice Cambien
Aug 2, 2007·Expert Opinion on Biological Therapy·Delphine CuchetAlberto L Epstein
May 31, 2007·Neoplasia : an International Journal for Oncology Research·Norman KockKhalid Shah
May 14, 2004·Neoplasia : an International Journal for Oncology Research·Shanta M MesserliChing-Hsuan Tung
Jul 27, 2011·Trends in Biotechnology·Christian E Badr, Bakhos A Tannous
Mar 26, 2009·Methods : a Companion to Methods in Enzymology·Yannic WaerzeggersAndreas H Jacobs
Dec 9, 2015·Angewandte Chemie·Eko Adi PrasetyantoLuisa De Cola
May 3, 2008·Luminescence : the Journal of Biological and Chemical Luminescence·Kemi CuiStephen T C Wong
Apr 8, 2006·The Journal of Gene Medicine·Lisa PikeSamuel Wang
Dec 8, 2009·The Journal of Pathology·Karen O'NeillAnnette T Byrne
May 4, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K Hansen, C Khanna
Nov 10, 2015·Chemical Reviews·Marcin PorebaMarcin Drag
May 18, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·William L ByrneMark Tangney

❮ Previous
Next ❯

Related Concepts

Related Feeds


Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis