Real-time imaging reveals endothelium-mediated leukocyte retention in LPS-treated lung microvessels.

Microvascular Research
Kathirvel KandasamyKaushik Parthasarathi

Abstract

Endotoxemia, a major feature of sepsis, is a common cause of acute lung injury and initiates rapid accumulation of leukocytes in the lung vasculature. Endothelial mechanisms that underlie this accumulation remain unclear, as current experimental models of endotoxemia are less suitable for targeted activation of the endothelium. Toward elucidating this, we used the isolated blood-perfused rat lung preparation. With a microcatheter inserted through a left atrial cannula, we cleared blood cells from a small lung region and then infused lipopolysaccharide (LPS) into microvessels. After a Ringer's wash to remove residual LPS, we infused fluorescently-labeled autologous leukocytes and imaged their transit through the treated microvessels. Image analysis revealed that leukocytes infused 90 min after LPS treatment were retained more in treated venules and capillaries than untreated vessels. Further, pretreatment with either the intercellular adhesion molecule-1 (ICAM-1) mAb or polymyxin-B blunted LPS-induced leukocyte retention in both microvessel segments. In addition, retention of leukocytes treated ex vivo with LPS in LPS-treated microvessels was higher compared to retention of untreated leukocytes. In situ immunofluorescence experi...Continue Reading

References

Oct 1, 1995·American Journal of Respiratory and Critical Care Medicine·G E KuhnleA E Goetz
Feb 1, 1997·The Journal of Clinical Investigation·N A DoyleC M Doerschuk
Apr 10, 1999·The Journal of Biological Chemistry·J C ChowF Gusovsky
Jan 5, 2000·American Journal of Respiratory and Critical Care Medicine·W M KueblerA E Goetz
May 10, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hideo IchimuraJahar Bhattacharya
Aug 27, 2005·American Journal of Respiratory Cell and Molecular Biology·Maimaiti T YimingSunita Bhattacharya
Feb 8, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Abdul BasitKlaus Ley
Jun 3, 2006·American Journal of Respiratory and Critical Care Medicine·Kazuo YoshidaClaire M Doerschuk
Aug 1, 2006·The Journal of Clinical Investigation·Kaushik ParthasarathiJahar Bhattacharya
Mar 3, 2009·Critical Care : the Official Journal of the Critical Care Forum·Alexander Melamed, Frank J Sorvillo
Mar 28, 2009·Critical Care Medicine·Sara E EricksonUNKNOWN NIH NHLBI ARDS Network
Nov 10, 2010·Critical Care : the Official Journal of the Critical Care Forum·Annette PloppaBoris Nohé
Feb 15, 2011·American Journal of Respiratory and Critical Care Medicine·Ashish K SharmaVictor E Laubach
Apr 27, 2011·The Journal of Clinical Investigation·David J RowlandsJahar Bhattacharya

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Citations

Sep 18, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Havovi ChichgerElizabeth O Harrington
May 9, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kaushik Parthasarathi
Nov 20, 2013·Annual Review of Physiology·Mark R Looney, Jahar Bhattacharya
Jan 22, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Heather MendenVenkatesh Sampath
Jul 15, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kathirvel KandasamyKaushik Parthasarathi

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