Real-time imaging reveals that lytic polysaccharide monooxygenase promotes cellulase activity by increasing cellulose accessibility

Biotechnology for Biofuels
Bo SongShi-You Ding

Abstract

The high cost of enzymes is one of the key technical barriers that must be overcome to realize the economical production of biofuels and biomaterials from biomass. Supplementation of enzyme cocktails with lytic polysaccharide monooxygenase (LPMO) can increase the efficiency of these cellulase mixtures for biomass conversion. The previous studies have revealed that LPMOs cleave polysaccharide chains by oxidization of the C1 and/or C4 carbons of the monomeric units. However, how LPMOs enhance enzymatic degradation of lignocellulose is still poorly understood. In this study, we combined enzymatic assays and real-time imaging using atomic force microscopy (AFM) to study the molecular interactions of an LPMO [TrAA9A, formerly known asTrCel61A) fromTrichoderma reesei] and a cellobiohydrolase I (TlCel7A fromT. longibrachiatum) with bacterial microcrystalline cellulose (BMCC) as a substrate. Cellulose conversion byTlCel7A alone was enhanced from 46 to 54% by the addition ofTrAA9A. Conversion by a mixture ofTlCel7A, endoglucanase, and β-glucosidase was increased from 79 to 87% using pretreated BMCC withTrAA9A for 72 h. AFM imaging demonstrated that individualTrAA9A molecules exhibited intermittent random movement along, across, and pene...Continue Reading

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Datasets Mentioned

BETA
CAA71999
AAA34212

Methods Mentioned

BETA
AFM

Software Mentioned

AFM
Nanoscope Analysis
Nanoscope
MassLynx

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