Real-Time In Situ Secondary Structure Analysis of Protein Monolayer with Mid-Infrared Plasmonic Nanoantennas

ACS Sensors
Dordaneh EtezadiHatice Altug

Abstract

Dynamic detection of protein conformational changes at physiological conditions on a minute amount of samples is immensely important for understanding the structural determinants of protein function in health and disease and to develop assays and diagnostics for protein misfolding and protein aggregation diseases. Herein, we experimentally demonstrate the capabilities of a mid-infrared plasmonic biosensor for real-time and in situ protein secondary structure analysis in aqueous environment at nanoscale. We present label-free ultrasensitive dynamic monitoring of β-sheet to disordered conformational transitions in a monolayer of the disease-related α-synuclein protein under varying stimulus conditions. Our experiments show that the extracted secondary structure signals from plasmonically enhanced amide I signatures in the protein monolayer can be reliably and reproducibly acquired with second derivative analysis for dynamic monitoring. Furthermore, by using a polymer layer we show that our nanoplasmonic approach of extracting the frequency components of vibrational signatures matches with the results attained from gold-standard infrared transmission measurements. By facilitating conformational analysis on small quantities of immo...Continue Reading

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Citations

Apr 26, 2019·Angewandte Chemie·Andreas TittlHatice Altug
May 28, 2021·The Journal of Physical Chemistry. B·Rodrigo Noriega
Feb 23, 2019·Analytical Chemistry·Kristen E WattsJeanne E Pemberton
Sep 9, 2020·The Journal of Physical Chemistry Letters·Emanuel Pfitzner, Joachim Heberle
Nov 19, 2020·Nano Letters·Debdatta RayOlivier J F Martin

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Methods Mentioned

BETA
circular dichroism
biosensing
surface plasmon resonances
biosensor
electron beam lithography
imaging techniques

Software Mentioned

Lumerical FDTD Solutions

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Alpha-synucleins are small proteins that are believed to restrict the mobility of synpatic vesicles and inhibit neurotransmitter release. Aggregation of these proteins have been linked to several types of neurodegenerative diseases including dementia with Lewy bodies and Parkinson's disease. Here is the latest research on α-synuclein aggregation.

Alpha-Synuclein Aggregation

Alpha-synucleins are small proteins that are believed to restrict the mobility of synpatic vesicles and inhibit neurotransmitter release. Aggregation of these proteins have been linked to several types of neurodegenerative diseases including dementia with Lewy bodies and Parkinson's disease. Here is the latest research on α-synuclein aggregation.