Real-time in vivo two-photon imaging study reveals decreased cerebro-vascular volume and increased blood-brain barrier permeability in chronically stressed mice

Scientific Reports
Sohee LeeMinah Suh

Abstract

Chronic stress disrupts brain homeostasis and adversely affects the cerebro-vascular system. Even though the effects of chronic stress on brain system have been extensively studied, there are few in vivo dynamic studies on the effects of chronic stress on the cerebro-vascular system. In this study, the effects of chronic stress on cerebral vasculature and BBB permeability were studied using in vivo two-photon (2p) microscopic imaging with an injection of fluorescence-conjugated dextran. Our real-time 2p imaging results showed that chronic stress reduced the vessel diameter and reconstructed vascular volume, regardless of vessel type and branching order. BBB permeability was investigated with two different size of tracers. Stressed animals exhibited a greater BBB permeability to 40-kDa dextran, but not to 70-kDa dextran, which is suggestive of weakened vascular integrity following stress. Molecular analysis revealed significantly higher VEGFa mRNA expression and a reduction in claudin-5. In summary, chronic stress decreases the size of cerebral vessels and increases BBB permeability. These results may suggest that the sustained decrease in cerebro-vascular volume due to chronic stress leads to a hypoxic condition that causes mol...Continue Reading

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Citations

Mar 5, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Christos TapeinosGianni Ciofani
Mar 8, 2020·Histochemistry and Cell Biology·Sanja Vignjević PetrinovićVladan Čokić
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Oct 13, 2021·Cellular and Molecular Neurobiology·Shusheng WuLongfei Du

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Methods Mentioned

BETA
confocal microscopy
ELISA
PCR

Software Mentioned

SPSS
QuantStudio 3 Real
Leica
Ethovision
Fiji
IMARIS
ImageJ

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