Real-time investigation of dynamic protein crystallization in living cells

Structural Dynamics
R SchönherrLars Redecke

Abstract

X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro.

References

Aug 1, 1986·The Journal of General Virology·G F Rohrmann
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G A KellerS Subramani
Feb 1, 1980·European Journal of Biochemistry·P M ColmanA Van Donkelaar
May 1, 1996·Microscopy Research and Technique·G Y FanL N Gastinel
Jun 19, 1998·Current Opinion in Structural Biology·G Dodson, D Steiner
Apr 27, 1999·Acta Crystallographica. Section D, Biological Crystallography·J R Luft, G T DeTitta
Aug 10, 2000·Revue Scientifique Et Technique·R C Jones
May 13, 2003·Microscopy Research and Technique·Marten VeenhuisIda J Van Der Klei
Dec 17, 2003·Methods in Enzymology·Mark WeselakRaymond C Stevens
Jul 1, 1996·Acta Crystallographica. Section D, Biological Crystallography·E ContiP Brick
Sep 13, 2005·Protein Science : a Publication of the Protein Society·Karin AnduleitDavid I Stuart
Jan 27, 2007·Current Opinion in Biotechnology·Tarik F MassoudSanjiv S Gambhir
Mar 3, 2007·Nature·Fasséli CoulibalyPeter Metcalf
Mar 16, 2007·Assay and Drug Development Technologies·Frank Fan, Keith V Wood
Feb 16, 2008·Histochemistry and Cell Biology·Michael Schrader, H Dariush Fahimi
Dec 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Fasséli CoulibalyPeter Metcalf
Dec 30, 2009·The Journal of Cell Biology·Shabeen AllyVladimir I Gelfand
Mar 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Douglas S AuldJames Inglese
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·James M Holton, Kenneth A Frankel
Apr 23, 2010·Molecular Biology of the Cell·Huajin WangRichard Lehner
Jul 28, 2010·The Journal of Cell Biology·Taro OhkawaMatthew D Welch
Feb 5, 2011·Nature·Henry N ChapmanJohn C H Spence
Feb 18, 2011·Acta Crystallographica. Section A, Foundations of Crystallography·Richard A KirianJohn C H Spence
Apr 6, 2011·The Journal of Biological Chemistry·Haruki HasegawaRandal R Ketchem
Apr 12, 2011·Nature Biotechnology·Roslyn M BillHorst Vogel
Jan 31, 2012·Nature Methods·Rudolf KoopmannMichael Duszenko
Apr 26, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Luís Pinto da Silva, Joaquim C G Esteves da Silva
Oct 2, 2012·Current Opinion in Structural Biology·Richard Neutze, Keith Moffat
May 2, 2013·Acta Crystallographica. Section D, Biological Crystallography·Thomas R M BarendsIlme Schlichting
Oct 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Irina V MajoulRainer Duden
Nov 26, 2013·Nature·Thomas R M BarendsIlme Schlichting
Jan 15, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Alexander McPherson, Jose A Gavira
Feb 15, 2014·Nature Communications·Uwe WeierstallVadim Cherezov
May 13, 2014·Acta Crystallographica. Section D, Biological Crystallography·Danny AxfordGeoff Sutton

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Citations

Jan 10, 2016·Archives of Biochemistry and Biophysics·José A Gavira
Jun 13, 2018·Biological Chemistry·Robert SchönherrLars Redecke
Oct 31, 2019·Traffic·Celestin N MudogoChristian Betzel
Jun 9, 2020·Acta Crystallographica. Section F, Structural Biology Communications·Nirupa NagaratnamJose M Martin-Garcia
Apr 7, 2016·Acta Crystallographica. Section D, Structural Biology·Marion BoudesFasséli Coulibaly
Dec 26, 2016·Histochemistry and Cell Biology·S NürnbergerD Barnewitz
Mar 5, 2021·Bio-protocol·Thomas L Li, Bianxiao Cui
Jul 3, 2021·Toxins·Guillaume TetreauJacques-Philippe Colletier
Jun 16, 2021·Experimental Cell Research·Chia-Chun ChangJi-Long Liu
Jun 13, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Haruki HasegawaFrederick Jacobsen

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Datasets Mentioned

BETA
KC152483.1

Methods Mentioned

BETA
X-ray
PCR
transfection
dot blot
Scanning electron microscopy
x-ray crystallography
light microscopy
transfections
x-ray diffraction
single-crystal diffraction

Software Mentioned

Andor Bioimaging
XFEL
Andor IQ2
Andor

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