Real-Time Investigation of Tuberculosis Transmission: Developing the Respiratory Aerosol Sampling Chamber (RASC)

PloS One
Robin WoodDigby F Warner

Abstract

Knowledge of the airborne nature of respiratory disease transmission owes much to the pioneering experiments of Wells and Riley over half a century ago. However, the mechanical, physiological, and immunopathological processes which drive the production of infectious aerosols by a diseased host remain poorly understood. Similarly, very little is known about the specific physiological, metabolic and morphological adaptations which enable pathogens such as Mycobacterium tuberculosis (Mtb) to exit the infected host, survive exposure to the external environment during airborne carriage, and adopt a form that is able to enter the respiratory tract of a new host, avoiding innate immune and physical defenses to establish a nascent infection. As a first step towards addressing these fundamental knowledge gaps which are central to any efforts to interrupt disease transmission, we developed and characterized a small personal clean room comprising an array of sampling devices which enable isolation and representative sampling of airborne particles and organic matter from tuberculosis (TB) patients. The complete unit, termed the Respiratory Aerosol Sampling Chamber (RASC), is instrumented to provide real-time information about the particula...Continue Reading

References

Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kaman ChanLalita Ramakrishnan
Dec 6, 2003·American Journal of Respiratory and Critical Care Medicine·Kevin P FennellyLeonid B Heifets
Feb 3, 2004·American Journal of Infection Control·Gwo-Hwa WanYing-Huang Tsai
Sep 19, 2008·PLoS Medicine·A Roderick EscombeCarlton A Evans
Apr 12, 2011·Tuberculosis·Ashwin S DharmadhikariEdward A Nardell
Feb 24, 2012·Journal of Clinical Microbiology·Alex van BelkumSonia Chatellier
Jul 14, 2012·Nature Reviews. Immunology·Joel D Ernst
Jul 17, 2012·American Journal of Respiratory and Critical Care Medicine·Kevin P FennellyJerrold J Ellner
Sep 10, 2013·Tuberculosis·Neus CaceresPere-Joan Cardona
Jan 7, 2015·American Journal of Respiratory and Critical Care Medicine·Jason R AndrewsThomas J Scriba
Feb 24, 2015·Journal of Theoretical Biology·Chacha M IssarowRobin Wood

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Citations

Nov 8, 2017·The Journal of Infectious Diseases·Richard D TurnerKevin P Fennelly
Jan 23, 2018·Gates Open Research·Benjamin PattersonRobin Wood
Sep 18, 2020·Journal of Clinical Medicine·Aysha ArshadVishwanath Venketaraman
Nov 8, 2017·The Journal of Infectious Diseases·Barun MathemaRobin Wood
Aug 11, 2020·BMC Infectious Diseases·Benjamin PattersonRobin Wood
Sep 4, 2020·PloS One·Benjamin PattersonRobin Wood
Oct 22, 2020·Journal of Breath Research·Dapeng ChenMichael McLoughlin
Dec 15, 2020·Tuberculosis·Benjamin PattersonRobin Wood
Feb 25, 2020·Tuberculosis·Nuno Rufino de SousaAntonio Gigliotti Rothfuchs
Jul 31, 2019·Accounts of Chemical Research·Timothy J de WetValerie Mizrahi
Oct 8, 2021·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Rachel K RedmannChad J Roy

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Methods Mentioned

BETA
PCR
sizing
genotyping
scanning electron microscopy
bronchoalveolar lavage

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