Real time measurement of stimulated dopamine release in the conscious rat using fast cyclic voltammetry: dopamine release is not observed during intracranial self stimulation

Journal of Neuroscience Methods
Z L KrukP Willner

Abstract

Fast cyclic voltammetry (FCV) was used to measure real time release of electrically stimulated endogenous dopamine in the nucleus accumbens (NAc) of conscious freely moving rats for up to 17 days. The method of electrode construction, implantation, electrical stimulation and recording of changes of extracellular dopamine concentration in the conscious rat are described. Rats trained on a continuous reinforcement schedule to perform intracranial self stimulation (ICSS) were implanted with electrodes for FCV. During ICSS, no faradaic signal was observed at an electrode implanted in the NAc. Decreasing the intensity of the stimulating current abolished ICSS, increasing the stimulating current disrupted ICSS. Operator delivered electrical stimulations using currents greater than those needed for ICSS yielded dopamine signals. It is concluded that during ICSS, sufficient dopamine does not reach the extracellular fluid space to yield a faradaic signal detectable by FCV.

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Citations

Nov 26, 2002·Behavioural Brain Research·Paul A Garris, George V Rebec
Dec 29, 2009·Nature Methods·Jeremy J ClarkPaul E M Phillips
Oct 3, 2002·Journal of Neurochemistry·R Mark Wightman, Donita L Robinson
Aug 7, 2013·Applied Physics Letters·Prabhu U ArumugamPaul A Garris
May 26, 2007·Brain Research·Matthew B LassenScott C Steffensen
May 6, 2015·Annual Review of Analytical Chemistry·Elizabeth S Bucher, R Mark Wightman
Jun 1, 2005·Behavioural Brain Research·Johanneke E van der HarstBerry M Spruijt
Jan 28, 2017·ACS Chemical Neuroscience·Nathan T RodebergR Mark Wightman
Jun 29, 2014·Pharmacological Reviews·S Stevens Negus, Laurence L Miller
Sep 23, 2003·Clinical Chemistry·Donita L RobinsonR Mark Wightman
Jun 23, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Changhoon SungSeongjun Park

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