Real-time monitoring of enzyme-free strand displacement cascades by colorimetric assays

Nanoscale
Ruixue DuanFan Xia

Abstract

The enzyme-free toehold-mediated strand displacement reaction has shown potential for building programmable DNA circuits, biosensors, molecular machines and chemical reaction networks. Here we report a simple colorimetric method using gold nanoparticles as signal generators for the real-time detection of the product of the strand displacement cascade. During the process the assembled gold nanoparticles can be separated, resulting in a color change of the solution. This assay can also be applied in complex mixtures, fetal bovine serum, and to detect single-base mismatches. These results suggest that this method could be of general utility to monitor more complex enzyme-free strand displacement reaction-based programmable systems or for further low-cost diagnostic applications.

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Citations

Dec 29, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Michael K LamJulianne Montfort Gibbs-Davis
Jan 28, 2016·Chemical Society Reviews·Ruixue DuanFan Xia
May 27, 2016·Chemical Communications : Chem Comm·Dongbao YaoHaojun Liang
Feb 23, 2020·Beilstein Journal of Nanotechnology·María Sanromán Iglesias, Marek Grzelczak
May 16, 2019·ACS Applied Materials & Interfaces·Bing WeiHaojun Liang

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