Real-time monitoring of mass-transport-related enzymatic reaction kinetics in a nanochannel-array reactor

Chemistry : a European Journal
Su-Juan LiHong-Yuan Chen

Abstract

To understand the fundamentals of enzymatic reactions confined in micro-/nanosystems, the construction of a small enzyme reactor coupled with an integrated real-time detection system for monitoring the kinetic information is a significant challenge. Nano-enzyme array reactors were fabricated by covalently linking enzymes to the inner channels of a porous anodic alumina (PAA) membrane. The mechanical stability of this nanodevice enables us to integrate an electrochemical detector for the real-time monitoring of the formation of the enzyme reaction product by sputtering a thin Pt film on one side of the PAA membrane. Because the enzymatic reaction is confined in a limited nanospace, the mass transport of the substrate would influence the reaction kinetics considerably. Therefore, the oxidation of glucose by dissolved oxygen catalyzed by immobilized glucose oxidase was used as a model to investigate the mass-transport-related enzymatic reaction kinetics in confined nanospaces. The activity and stability of the enzyme immobilized in the nanochannels was enhanced. In this nano-enzyme reactor, the enzymatic reaction was controlled by mass transport if the flux was low. With an increase in the flux (e.g., >50 microL min(-1)), the enzy...Continue Reading

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Citations

Jan 15, 2014·Biosensors & Bioelectronics·Jiachao YuSongqin Liu
Jan 17, 2016·Analytica Chimica Acta·Juan QiaoMyeong Hee Moon
May 28, 2016·Chemical Communications : Chem Comm·Jusheng LuSongqin Liu
Apr 11, 2020·Chemistry : a European Journal·Yunfeng HanHaibing Li
Feb 9, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Chen WangXing-Hua Xia
Feb 23, 2013·Lab on a Chip·Chen WangXing-Hua Xia
Sep 30, 2014·Chemical Communications : Chem Comm·Jusheng LuSongqin Liu
Nov 17, 2011·ChemSusChem·Vasile SmuleacDibakar Bhattacharyya
Jun 2, 2012·Physical Chemistry Chemical Physics : PCCP·Hong-Li GaoXing-Hua Xia
Nov 14, 2018·ACS Applied Materials & Interfaces·Ling JiangSongqin Liu
Jul 19, 2014·Analytical Chemistry·Jiachao YuSongqin Liu

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