Real-time monitoring of stress erythropoiesis in vivo using Gata1 and beta-globin LCR luciferase transgenic mice

Blood
Mikiko SuzukiMasayuki Yamamoto

Abstract

Erythroid progenitors have the potential to proliferate rapidly in response to environmental stimuli. This process is referred to as stress erythropoiesis, with erythropoietin (EPO) playing central roles in its promotion. In this study, we wanted to elucidate the molecular mechanisms governing the regulation of stress erythropoiesis and the maintenance of red-cell homeostasis. This was achieved by our development of a noninvasive real-time monitoring system for erythropoiesis using transgenic mouse lines expressing luciferase under the control of the mouse Gata1 hematopoietic regulatory domain (G1-HRD-luc) or human beta-globin locus control region (Hbb-LCR-luc). Optical bioluminescence images revealed that the luciferase was specifically expressed in spleen and bone marrow and was induced rapidly in response to anemia and hypoxia stimuli. The G1-HRD-luc activity tracked the emergence and disappearance of proerythroblast-stage progenitors, whereas the Hbb-LCR-luc activity tracked erythroblasts and later stage erythroid cells. Increased plasma EPO concentration preceded an increase in G1-HRD-luc, supporting our contention that EPO acts as the key upstream signal in stress erythropoiesis. Hence, we conclude that G1-HRD-luc and Hbb...Continue Reading

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Citations

Jun 4, 2013·Nature Communications·Shun YamazakiMasayuki Yamamoto
Feb 23, 2008·Proceedings. Biological Sciences·Victoria C BarclayAndrew F Read
Mar 28, 2008·Proceedings. Biological Sciences·Rustom AntiaJacobus C de Roode
Mar 6, 2007·Development, Growth & Differentiation·Hideki Masaki, Hiroyuki Ide
Dec 24, 2008·Molecular and Cellular Biology·Mikiko SuzukiMasayuki Yamamoto
Sep 16, 2015·Proceedings of the National Academy of Sciences of the United States of America·Nadine Keleku-LukweteMasayuki Yamamoto
Jul 9, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Nadeem YaqoobThorsten Schwerte
Nov 5, 2011·Journal of Cellular and Molecular Medicine·Feng-Lin ZhangJun-Wu Zhang
Aug 19, 2015·Molecular and Cellular Biology·Makiko HayashiMasayuki Yamamoto
Aug 6, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Mikiko SuzukiMasayuki Yamamoto

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