Real-time monitoring of the cell agglutination process with a quartz crystal microbalance

Analytical Biochemistry
Liang TanQingji Xie

Abstract

The real-time monitoring of the agglutination process of human hepatic normal cells (L-02) at the quartz crystal microbalance (QCM) gold (Au) electrode was performed. Two lectins, concanavalin A (Con A) and wheat germ agglutinin (WGA), induced the cell agglutination, resulting in the different Deltaf(0) and DeltaR(1) responses from those caused by the normal cell attachment and growth. The cell-Con A-cell aggregates had higher affinity for the Au substrate due to the excellent adsorption ability of Con A, which was revealed by increased Deltaf(0) and DeltaR(1) shifts and the obvious mass effect of QCM. In contrast, the lower adsorption ability of cell-WGA-cell aggregates was related to the same characteristic of WGA, presenting the decreased Deltaf(0) and DeltaR(1) responses and the time-extended adhesion phase. Parallel microscopic observation experiments were also carried out and exhibited comparable results. The Deltaf(0) responses during the processes of cell growth and cell agglutination were analyzed using the equations Deltaf(0)=alpha(0)+alpha(1)e(-t/tau(1))+alpha(2)e(-t/tau(2))+alpha(3)e(-t/tau(3)) and Deltaf(0)=alpha(0)+alpha(1)e(-t/tau(1))+alpha(2)e(-t/tau(2)), respectively. Furthermore, the current work proved that t...Continue Reading

References

Oct 13, 1989·Science·N Sharon, H Lis
Dec 1, 1993·Analytical Chemistry·J RedepenningR Bizios
Jan 12, 1999·Analytical Biochemistry·O E GalaninaN V Bovin
Jul 25, 2003·Analytical and Bioanalytical Chemistry·Carine Galli MarxerLouis Schlapbach
Oct 14, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Katsuhiko SatoJun-ichi Anzai
Jan 25, 2005·Biomaterials·Delphine Le Guillou-BuffelloVéronique Migonney
Jul 14, 2005·Journal of the American Chemical Society·Ting ZhengLloyd M Smith
Apr 6, 2007·Analytical Chemistry·Vanessa Heitmann, Joachim Wegener

❮ Previous
Next ❯

Citations

Oct 15, 2011·Cellular and Molecular Life Sciences : CMLS·Michael Saitakis, Electra Gizeli
Apr 28, 2011·Journal of Biochemical and Molecular Toxicology·Glaucia Veríssimo Faheina-MartinsDemetrius Antonio Machado Araujo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.