Real-Time Monitoring of Tricarboxylic Acid Metabolites in Exhaled Breath

Analytical Chemistry
Alberto Tejero RioserasPablo Martinez-Lozano Sinues

Abstract

The tricarboxylic acid (TCA) cycle is one of the most important metabolic pathway for cellular respiration in aerobic organisms. It provides and collects intermediates for many other interconnecting pathways and acts as a hub connecting metabolism of carbohydrates, fatty acids, and amino acids. Alteration in intracellular levels of its intermediates has been linked with a wide range of illnesses ranging from cancer to cellular necrosis or liver cirrhosis. Therefore, there exists an intrinsic interest in monitoring such metabolites. Our goal in this study was to evaluate whether, at least the most volatile metabolites of the TCA cycle, could be detected in breath in vivo and in real time. We used secondary electrospray ionization coupled with high-resolution mass spectrometry (SESI-HRMS) to conduct this targeted analysis. We enrolled six healthy individuals who provided full exhalations into the SESI-HRMS system at different times during 3 days. For the first time, we observed exhaled compounds that appertain to the TCA cycle: fumaric, succinic, malic, keto-glutaric, oxaloacetic, and aconitic acids. We found high intraindividual variability and a significant overall difference between morning and afternoon levels for malic acid,...Continue Reading

References

Jan 1, 1996·Rapid Communications in Mass Spectrometry : RCM·D Smith, P Spanel
Jan 5, 2007·Nucleic Acids Research·David S WishartLori Querengesser
Sep 20, 2007·European Journal of Mass Spectrometry·P Spanĕl, D Smith
Sep 30, 2008·Analytical Chemistry·Pablo Martínez-Lozano, Juan Fernández de la Mora
Nov 18, 2008·Journal of the American Society for Mass Spectrometry·Pablo Martínez-LozanoJuan Fernández de la Mora
Nov 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ferdia A GallagherKevin M Brindle
Apr 15, 2010·Rapid Communications in Mass Spectrometry : RCM·Kseniya DryahinaP Spanel
Jun 1, 2009·Journal of Breath Research·Jens HerbigArmin Hansel
Jun 23, 2011·Journal of Visualized Experiments : JoVE·Heather D BeanJane E Hill
Dec 20, 2011·Regulatory Toxicology and Pharmacology : RTP·Harrie E BuistWouter H J Vaes
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Robert DallmannSteven A Brown
Aug 14, 2012·International Journal of Cell Biology·Simone Cardaci, Maria Rosa Ciriolo
Sep 14, 2012·Analytical Chemistry·G Vidal-de-MiguelJ Blanco
Oct 12, 2012·Nature Biotechnology·Matthew C ChambersParag Mallick
Nov 20, 2012·Nucleic Acids Research·David S WishartAugustin Scalbert
Dec 4, 2012·Analytical Chemistry·Pablo Martinez-Lozano SinuesRenato Zenobi
Apr 11, 2013·PloS One·Pablo Martinez-Lozano SinuesRenato Zenobi
Aug 14, 2013·Rapid Communications in Mass Spectrometry : RCM·Kseniya DryahinaDavid Smith
Feb 15, 2014·Cell Cycle·Javier A MenendezJorge Joven
Mar 1, 2014·European Journal of Mass Spectrometry·Patrik Spanel, David Smith
Dec 30, 2014·PloS One·Pablo Martinez-Lozano SinuesRobert Dallmann
Oct 28, 2015·The Journal of Physical Chemistry. a·Matthew J AerneckeRoderick R Kunz
Jan 7, 2016·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·T LomonacoF Di Francesco
May 18, 2016·Journal of Breath Research·David SmithPatrik Španěl
Sep 15, 2016·Nature Communications·Mélissa CarbonneauFrédérick A Mallette
Dec 3, 2016·Bioresources and Bioprocessing·Xingxing JianQiang Hua
Apr 14, 2017·Respiratory Research·Charmion Cruickshank-QuinnNichole Reisdorph
Sep 1, 2017·Analytical Chemistry·Martin Thomas GauggRenato Zenobi
Sep 14, 2017·Journal of Breath Research·Martin T GauggPablo Martinez-Lozano Sinues
Nov 10, 2017·Analytical Chemistry·D ZamoraJ Fernández de la Mora
Feb 8, 2018·Journal of Breath Research·Kapil Dev SinghPablo M-L Sinues

❮ Previous
Next ❯

Citations

Nov 23, 2019·Rapid Communications in Mass Spectrometry : RCM·Regina Brůhová MichalčíkováPatrik Španěl
Jan 27, 2019·Respirology : Official Journal of the Asian Pacific Society of Respirology·Martin Thomas GauggMalcolm Kohler
Mar 25, 2021·Journal of Breath Research·Natalia DrabińskaBen De Lacy Costello
Oct 9, 2019·Chemical Reviews·Tobias BrudererRenato Zenobi
Oct 18, 2019·Journal of Proteome Research·Xiaoling ZangFacundo M Fernández

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.