Real-time NMR monitoring of biological activities in complex physiological environments

Current Opinion in Structural Biology
Matthew J SmithMitsuhiko Ikura

Abstract

Biological reactions occur in a highly organized spatiotemporal context and with kinetics that are modulated by multiple environmental factors. To integrate these variables in our experimental investigations of 'native' biological activities, we require quantitative tools for time-resolved in situ analyses in physiologically relevant settings. Here, we outline the use of high-resolution NMR spectroscopy to directly observe biological reactions in complex environments and in real-time. Specifically, we discuss how real-time NMR (RT-NMR) methods have delineated insights into metabolic processes, post-translational protein modifications, activities of cellular GTPases and their regulators, as well as of protein folding events.

References

Mar 18, 2004·Journal of the American Chemical Society·I G DenisovS G Sligar
Feb 26, 2008·Nature Structural & Molecular Biology·Philipp SelenkoGerhard Wagner
Jan 30, 2009·Science Signaling·Christopher B MarshallVuk Stambolic
Dec 19, 2009·The Journal of Biological Chemistry·Geneviève M C Gasmi-SeabrookMitsuhiko Ikura
Sep 24, 2010·The Journal of Biological Chemistry·Vanessa BuosiCarine van Heijenoort
Oct 5, 2010·Journal of the American Chemical Society·Stamatios LiokatisPhilipp Selenko
Apr 24, 2012·Journal of the American Chemical Society·François-Xavier TheilletPhilipp Selenko
Jul 17, 2012·Nature Structural & Molecular Biology·Stamatios LiokatisPhilipp Selenko
Sep 27, 2012·Journal of Biomolecular NMR·Francois-Xavier TheilletPhilipp Selenko
Nov 23, 2012·Journal of the American Chemical Society·Florence CordierNicolas Wolff
Feb 13, 2013·Journal of the American Chemical Society·Alexander S MaltsevAd Bax
Feb 16, 2013·Journal of the American Chemical Society·Mohammad T Mazhab-JafariMitsuhiko Ikura
Mar 5, 2013·Cell·Jeremy E Purvis, Galit Lahav
Mar 5, 2013·Nature Chemical Biology·Lucia BanciA Radu Aricescu
Mar 15, 2013·The Journal of Biological Chemistry·Stephanie Hoffmann-ThomsFranz X Schmid
Mar 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Matthew J SmithMitsuhiko Ikura
Jun 8, 2013·Chembiochem : a European Journal of Chemical Biology·Irene AmataMiquel Pons
Jul 17, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Enrico Rennella, Bernhard Brutscher
Sep 11, 2013·Nature Communications·Myriam M ChaumeilSabrina M Ronen
Jan 18, 2014·Journal of Biomolecular NMR·Maxim MayzelVladislav Y Orekhov
Jan 21, 2014·Nature Chemical Biology·Matthew J Smith, Mitsuhiko Ikura
Apr 26, 2014·ACS Chemical Biology·E Neil G Marsh, Yuta Suzuki
Aug 2, 2014·Analytical Chemistry·Mathilde H LercheSebastian Meier
Aug 15, 2014·ACS Chemical Biology·Sujan S ShekhawatEric R Strieter
Nov 5, 2014·Nature Chemical Biology·Wade BorcherdsGary W Daughdrill

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Citations

Aug 31, 2016·Progress in Nuclear Magnetic Resonance Spectroscopy·Ayako FurukawaKenji Sugase
Oct 19, 2016·Nature Chemical Biology·Gleb G MironovMaxim V Berezovski
Mar 3, 2017·IUCrJ·Marc Baldus
Jul 1, 2017·The Journal of Physical Chemistry. B·Michael FeigYuji Sugita
Aug 2, 2018·Cellular and Molecular Life Sciences : CMLS·Alexander V FoninVladimir N Uversky
Mar 17, 2019·International Journal of Molecular Sciences·Philipp Selenko
Apr 25, 2017·Science Advances·Pavel MacekJerome Boisbouvier
Jun 8, 2017·Cellular and Molecular Life Sciences : CMLS·Vladimir N Uversky
Jul 31, 2019·Nature Communications·Pierre BarraudCarine Tisné
May 2, 2020·Acta Crystallographica. Section D, Structural Biology·John H Beale
Jan 20, 2019·International Journal of Molecular Sciences·Amit KumarJochen Balbach
Jan 6, 2019·International Journal of Molecular Sciences·CongBao Kang
Jun 6, 2020·Chemistry : a European Journal·Carolina Sánchez-LópezAndres Binolfi
Jul 31, 2019·Journal of Magnetic Resonance·Gregg Siegal, Philipp Selenko
Nov 5, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Imran KhanJohn P O'Bryan
Aug 18, 2020·Chembiochem : a European Journal of Chemical Biology·Anass ChikiHilal A Lashuel
Mar 5, 2021·Emerging Topics in Life Sciences·Hong ZhangSarah Perrett
Apr 9, 2021·Journal of Biochemistry·Kazuhiko YamasakiHiroto Suematsu
May 13, 2015·Journal of the American Chemical Society·Rossukon ThongwichianPhilipp Selenko

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