Real-time PCR approach for detection of environmental sources of Campylobacter strains colonizing broiler flocks.

Applied and Environmental Microbiology
A RidleyD G Newell

Abstract

Reducing colonization of poultry flocks by Campylobacter spp. is a key strategy in the control and prevention human campylobacteriosis. Horizontal transmission of campylobacters, from in and around the farm, is the presumed route of flock colonization. However, the identification and prioritization of sources are confounded by the ubiquitous nature of these organisms in the environment, their poor rates of recovery by standard culture methods, and the need for cost-effective and timely methods for strain-specific comparison. A real-time PCR screening test for the strain-specific detection of campylobacters in environmental samples has been developed to address this issue. To enable this approach, fluorescently labeled PCR oligonucleotide probes suitable for a LightCycler-based assay were designed to match a highly variable DNA segment within the flaA short variable region (SVR) of Campylobacter jejuni or C. coli. The capacity of such probes to provide strain-specific tools was investigated by using bacterial cultures and spiked and naturally contaminated poultry fecal and environmental samples. The sensitivity of two representative probes was estimated, by using two different C. jejuni strains, to be 1.3 x 10(2) to 3.7 x 10(2) ...Continue Reading

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Citations

Mar 26, 2010·Epidemiology and Infection·T L StuartE Galanis
Dec 3, 2011·Vector Borne and Zoonotic Diseases·David HermansFreddy Haesebrouck
Oct 11, 2011·Applied and Environmental Microbiology·D G NewellV M Allen
Nov 3, 2010·Applied and Environmental Microbiology·Anne M RidleyVivien M Allen
Jun 21, 2012·Zoonoses and Public Health·Y SasakiY Yamada
Jul 14, 2010·Veterinary Microbiology·Thomas AlterGerhard Glünder
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Feb 1, 2021·Poultry Science·Pongthorn Pumtang-OnThiru Vanniasinkam
May 29, 2021·Analytical and Bioanalytical Chemistry·Nadja E Solis-MarcanoCarlos R Cabrera

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