Due to the severity of the food-borne infection listeriosis, strict legislation governs the detectable and permissible limits at which Listeria monocytogenes is permitted in foods. These requirements, coupled with the ubiquitous nature of L. monocytogenes strains and the potential for epidemic outbreaks, mean that the pathogen can devastate affected sectors of the food industry. Although almost all L. monocytogenes strains have the potential to cause listeriosis, those implicated in the vast majority of epidemics belong to a subset of strains belonging to evolutionary lineage I. It has been established that a significant proportion of these strains, including those implicated in the majority of outbreaks, produce an additional hemolysin, designated listeriolysin S (LLS), which may be responsible for the enhanced virulence of these strains. In order to ultimately establish this definitively, it is important to first be able to rapidly discriminate between LLS-positive and -negative strains. Here, after essential genes within the LLS-encoding cluster, Listeria pathogenicity island 3, were identified by deletion mutagenesis, a real-time PCR assay which targets one such gene, llsX, was developed as a means of identifying LLS-positi...Continue Reading
Distribution of serovars of Listeria monocytogenes isolated from different categories of patients with listeriosis
Aspects of the epidemiology of human Listeria monocytogenes infections in Britain 1967-1984; the use of serotyping and phage typing
A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli
A family of bacteriocin ABC transporters carry out proteolytic processing of their substrates concomitant with export
From peptide precursors to oxazole and thiazole-containing peptide antibiotics: microcin B17 synthase
The inlA gene of Listeria monocytogenes LO28 harbors a nonsense mutation resulting in release of internalin
Comparative genetic characterization of Listeria monocytogenes isolates from human and animal listeriosis cases
Quantitative detection of Listeria monocytogenes and Listeria innocua by real-time PCR: assessment of hly, iap, and lin02483 targets and AmpliFluor technology.
Mutational analysis of the group A streptococcal operon encoding streptolysin S and its virulence role in invasive infection
Rapid quantitative detection of, Listeria monocytogenes in salmon products: evaluation of pre-real-time PCR strategies
Select Listeria monocytogenes subtypes commonly found in foods carry distinct nonsense mutations in inlA, leading to expression of truncated and secreted internalin A, and are associated with a reduced invasion phenotype for human intestinal epithelial cells
Detection of Listeria monocytogenes in food using a combined enrichment/real-time PCR method targeting the prfA gene
Recurrent and sporadic Listeria monocytogenes contamination in alheiras represents considerable diversity, including virulence-attenuated isolates
Nonsense-mutated inlA and prfA not widely distributed in Listeria monocytogenes isolates from ready-to-eat seafood products in Japan
Rapid real-time PCR detection of Listeria monocytogenes in enriched food samples based on the ssrA gene, a novel diagnostic target
Listeriolysin S, a novel peptide haemolysin associated with a subset of lineage I Listeria monocytogenes.
inlA premature stop codons are common among Listeria monocytogenes isolates from foods and yield virulence-attenuated strains that confer protection against fully virulent strains.
Development and implementation of a multiplex single-nucleotide polymorphism genotyping assay for detection of virulence-attenuating mutations in the Listeria monocytogenes virulence-associated gene inlA
Rapid detection of Listeria monocytogenes in food using culture enrichment combined with real-time PCR
Prevalence and challenge tests of Listeria monocytogenes in Belgian produced and retailed mayonnaise-based deli-salads, cooked meat products and smoked fish between 2005 and 2007
Quantification of Listeria monocytogenes in minimally processed leafy vegetables using a combined method based on enrichment and 16S rRNA real-time PCR
The microbiological safety of ready-to-eat specialty meats from markets and specialty food shops: a UK wide study with a focus on Salmonella and Listeria monocytogenes
Attribution of human Listeria monocytogenes infections in England and Wales to ready-to-eat food sources placed on the market: adaptation of the Hald Salmonella source attribution model
Characterization and antimicrobial resistance of Listeria monocytogenes isolated from retail beef meat in Poland
Prevalence, characterization, and antimicrobial resistance of Listeria monocytogenes isolates from bovine hides and carcasses.
Evaluation of immunochromatography for the rapid and specific identification of Listeria monocytogenes from food
Analysis of Multilocus Sequence Typing and Virulence Characterization of Listeria monocytogenes Isolates from Chinese Retail Ready-to-Eat Food
Real-time PCR detection of Listeria monocytogenes in infant formula and lettuce following macrophage-based isolation and enrichment
Identification of the minimal cytolytic unit for streptolysin S and an expansion of the toxin family
Magnetic bead based immuno-detection of Listeria monocytogenes and Listeria ivanovii from infant formula and leafy green vegetables using the Bio-Plex suspension array system
Genomic Diversity of Listeria monocytogenes Isolated from Clinical and Non-Clinical Samples in Chile
Prevalence and molecular characteristics of Listeria monocytogenes in cooked products and its comparison with isolates from listeriosis cases
Genetic characteristics and virulence of Listeria monocytogenes isolated from fresh vegetables in China
The Effect of a Commercially Available Bacteriophage and Bacteriocin on Listeria monocytogenes in Coleslaw
Characterization of Virulence and Persistence Abilities of Listeria monocytogenes Strains Isolated from Food Processing Premises
Virulence factors and resistance to antimicrobials in Listeria monocytogenes serotype 1/2c isolated from food
Isolation of phage-display library-derived scFv antibody specific to Listeria monocytogenes by a novel immobilized method
Listeriolysin S: A bacteriocin from epidemic Listeria monocytogenes strains that targets the gut microbiota
Heterogeneity, Characteristics, and Public Health Implications of Listeria monocytogenes in Ready-to-Eat Foods and Pasteurized Milk in China
Prevalence, Potential Virulence, and Genetic Diversity of Listeria monocytogenes Isolates From Edible Mushrooms in Chinese Markets
Occurrence, Antibiotic Resistance, and Population Diversity of Listeria monocytogenes Isolated From Fresh Aquatic Products in China
Whole-Genome Sequencing-Based Characterization of 100 Listeria monocytogenes Isolates Collected from Food Processing Environments over a Four-Year Period
Isolation, Potential Virulence, and Population Diversity of Listeria monocytogenes From Meat and Meat Products in China
Prevalence and transmission characteristics of Listeria species from ruminants in farm and slaughtering environments in China.
Interference of the acid stress on the expression of llsX by Listeria monocytogenes pathogenic island 3 (LIPI-3) variants.
Listeriolysin S: A bacteriocin from Listeria monocytogenes that induces membrane permeabilization in a contact-dependent manner.
CRISPR & Staphylococcus
CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.