Real-time PCR assay to differentiate Listeriolysin S-positive and -negative strains of Listeria monocytogenes

Applied and Environmental Microbiology
Evelyn M ClaytonR Paul Ross

Abstract

Due to the severity of the food-borne infection listeriosis, strict legislation governs the detectable and permissible limits at which Listeria monocytogenes is permitted in foods. These requirements, coupled with the ubiquitous nature of L. monocytogenes strains and the potential for epidemic outbreaks, mean that the pathogen can devastate affected sectors of the food industry. Although almost all L. monocytogenes strains have the potential to cause listeriosis, those implicated in the vast majority of epidemics belong to a subset of strains belonging to evolutionary lineage I. It has been established that a significant proportion of these strains, including those implicated in the majority of outbreaks, produce an additional hemolysin, designated listeriolysin S (LLS), which may be responsible for the enhanced virulence of these strains. In order to ultimately establish this definitively, it is important to first be able to rapidly discriminate between LLS-positive and -negative strains. Here, after essential genes within the LLS-encoding cluster, Listeria pathogenicity island 3, were identified by deletion mutagenesis, a real-time PCR assay which targets one such gene, llsX, was developed as a means of identifying LLS-positi...Continue Reading

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Citations

Aug 9, 2011·Nature Reviews. Microbiology·Evelyn M MolloyR Paul Ross
Mar 13, 2014·BMC Microbiology·Evelyn M ClaytonPaul R Ross
Nov 21, 2015·Journal of Food Science·Esmond B Nyarko, Catherine W Donnelly
May 4, 2016·Proceedings of the National Academy of Sciences of the United States of America·Juan J QueredaJavier Pizarro-Cerda
Mar 4, 2017·Chemical Reviews·Brandon J BurkhartDouglas A Mitchell
Apr 9, 2014·Antimicrobial Agents and Chemotherapy·Harsh MathurR Paul Ross
Mar 20, 2019·Current Microbiology·Rodolfo Erik Vilchis-RangelOscar Rodolfo Rodas-Suárez
Feb 12, 2020·Journal of Food Protection·Elżbieta MaćkiwJacek Postupolski
May 31, 2016·Journal of Applied Microbiology·T GelbíčováR Karpíšková
Dec 21, 2020·International Journal of Food Microbiology·Elżbieta MaćkiwJacek Postupolski
Apr 26, 2020·Food Research International·Rafaela de Melo TavaresLuís Augusto Nero
Feb 11, 2015·ACS Chemical Biology·Tucker MaxsonDouglas A Mitchell
Oct 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jazmín Meza-TorresJavier Pizarro-Cerdá

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