Abstract
Quantification Hepatitis B virus (HBV) DNA plays a critical role in the management of chronic HBV infections. However, HBV is a DNA virus with high levels of genetic variation, and drug-resistant mutations have emerged with the use of antiviral drugs. If a mutation caused a sequence mismatched in the primer or probe of a commercial DNA quantification kit, this would lead to an underestimation of the viral load of the sample. The aim of this study was to determine whether commercial kits, which use only one pair of primers and a single probe, accurately quantify the HBV DNA levels and to develop an improved duplex real-time PCR assay. We developed a new duplex real-time PCR assay that used two pairs of primers and two probes based on the conserved S and C regions of the HBV genome. We performed HBV DNA quantitative detection of HBV samples and compared the results of our duplex real-time PCR assays with the COBAS TaqMan HBV Test version 2 and Daan real-time PCR assays. The target region of the discordant sample was amplified, sequenced, and validated using plasmid. The results of the duplex real-time PCR were in good accordance with the commercial COBAS TaqMan HBV Test version 2 and Daan real-time PCR assays. We showed that two ...Continue Reading
References
Jun 1, 1989·Virology·R Girones, R H Miller
Sep 1, 1995·Journal of Virology·S GüntherH Will
Sep 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G A TipplesD L Tyrrell
Jan 21, 2000·The Journal of General Virology·L StuyverR Rossau
Mar 9, 2002·Nucleic Acids Research·Ian M MackayAndreas Nitsche
Mar 22, 2005·Journal of Medical Virology·Takao ShibayamaHiroaki Okamoto
Apr 20, 2006·Journal of Medical Virology·José M Echevarría, Ana Avellón
Jul 11, 2006·Journal of Clinical Microbiology·Magnus LindhGunnar Norkrans
Jan 19, 2007·Journal of Clinical Microbiology·Tiziano AlliceValeria Ghisetti
Dec 22, 2007·Journal of Hepatology·Giovanna FattovichFrancesco Donato
Mar 4, 2008·Intervirology·Jie HuangJin-Ming Li
Sep 19, 2008·Journal of Clinical Microbiology·Johannes VermehrenChristoph Sarrazin
Feb 6, 2009·Journal of Clinical Microbiology·Klaus KornJosef Eberle
Apr 17, 2009·Journal of Clinical Microbiology·Stéphane ChevaliezJean-Michel Pawlotsky
Sep 5, 2009·Vaccine·Xiaofeng LiangYu Wang
Jun 10, 2010·Virology Journal·Shuang Meng, Jinming Li
Feb 25, 2011·Journal of Public Health·Patrick KirwanLisa Brant
May 17, 2011·Virology Journal·Shipeng SunJinming Li
Mar 23, 2012·Journal of Hepatology·UNKNOWN European Association For The Study Of The Liver
Apr 28, 2012·Gastroenterology·Stéphane ChevaliezJean-Michel Pawlotsky
Feb 7, 2013·Journal of Viral Hepatitis·S GhoshS Datta
Nov 18, 2015·Journal of Medical Virology·Krishna ShahIndira Hewlett
Citations
Sep 29, 2018·Genes·Emilia Hadziyannis, Andreas Laras
Jul 25, 2019·Journal of Viral Hepatitis·Dong Hyun SinnMoon Seok Choi
Jul 17, 2020·Journal of Clinical Microbiology·Laure BoizeauPierre Cappy
May 1, 2020·BioMed Research International·Salma MadihiAbdelouaheb Benani
Aug 8, 2019·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Chao LiuLunan Wang
Aug 4, 2020·Royal Society Open Science·Kashif Aziz Khan, Peter Cheung
May 11, 2020·Biotechnology and Applied Biochemistry·Ting-Ting YiYan Cai
Jan 21, 2021·BMC Infectious Diseases·Xianlin YeJinfeng Zeng
Feb 8, 2021·Journal of Virological Methods·Bashan ZhangAijiao Ding
Jun 20, 2021·Molecular and Cellular Probes·Kabilan ManiSankarganesh Jeyaraj
Nov 29, 2021·Archives of Virology·Leonardo A SilvaDaniel M P Ardisson-Araújo