Real-Time PCR based test for the early diagnosis of Haplosporidium pinnae affecting fan mussel Pinna nobilis

PloS One
Monserrat López-SanmartínJosé Ignacio Navas

Abstract

Noble pen shell or fan mussel, Pinna nobilis Linnaeus (1758), protected since 1992, was incorporated into the Spanish Catalogue of Threatened Species (Category: Vulnerable, Royal Decree 139/2011). The status is presently in the process of being catalogued as critically endangered, pending approval by Spanish Government (https://www.mapama.gob.es/es/biodiversidad/participacion-publica/Borrador_OM_situacion_critica.aspx). The International Union for the Conservation of Nature (IUCN) alerted the countries of the Mediterranean basin to the "emergent situation" due to serious mortality events suffered by the fan mussel, putting it in serious risk of extinction. Thus, emergency actions have been implemented by Spanish authorities in which several research institutes from all over the country are involved. The parasite, Haplosporidium pinnae, was recently characterized by histology, TEM, SEM and molecular biology techniques and it was considered responsible for the mass mortality of P. nobilis in the Mediterranean Sea. In this context, the aim of this study has been to develop species-specific quantitative PCR (qPCR) protocol carrying out a fast, specific and effective molecular diagnose of H. pinnae. In this sense, the detection limi...Continue Reading

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Citations

Dec 11, 2020·Marine Environmental Research·Patricia PradoJosé Rafael García-March
Apr 27, 2021·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·İbrahim Ender KüniliUğur Altinağaç
May 4, 2021·Journal of Clinical Laboratory Analysis·Zhikang YuZhixiong Zhong

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Datasets Mentioned

BETA
MK142774
MK142779

Methods Mentioned

BETA
PCR
PCRs
electrophoresis
biopsies
biopsy

Software Mentioned

Clustal
BLAST
Primer
Basic Local Alignment Search Tool
SPSS Statistics
ChromasPro

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