Real-time PCR detection of gyrA and parC mutations in Streptococcus pneumoniae.

Antimicrobial Agents and Chemotherapy
S PageS Le Hello

Abstract

Fluoroquinolone resistance in Streptococcus pneumoniae mainly involves stepwise mutations predominantly in the parC and gyrA genes. We have developed a single-run real-time PCR assay for detection of the four most common mutations in the quinolone resistance-determining regions of these genes. This assay provides a useful tool for both clinical and epidemiological use.

References

Aug 1, 1987·European Journal of Clinical Microbiology·P C Appelbaum
Mar 30, 2002·Journal of Clinical Microbiology·Kevin Barratt, John F Mackay
Apr 23, 2003·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Stephen H GillespieAnne Dickens
Oct 2, 2003·Emerging Infectious Diseases·Emilio Pérez-TralleroJulián Larruskain
Jan 27, 2006·Antimicrobial Agents and Chemotherapy·Emmanuelle VaronUNKNOWN Groupe des Observatoires de la Résistance du Pneumocoque
Nov 1, 2006·Emerging Infectious Diseases·Mathias W R PletzKeith P Klugman
May 23, 2008·International Journal of Antimicrobial Agents·S Le HelloB Garin

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