Real-time PCR detection of Moritella viscosa, the likely causal agent of winter-ulcer in Atlantic salmon Salmo salar and rainbow trout Oncorhynchus mykiss

Diseases of Aquatic Organisms
S GroveD J Colquhoun

Abstract

We report the development of a real-time PCR protocol for specific detection of Moritella viscosa. This bacterium is considered to be the main aetiological agent in development of winter-ulcer, a disease severely affecting salmonid aquaculture in Norway. From a newly elaborated draft version of the genome of M. viscosa, the tonB gene sequence was selected as a suitable basis for the development of the real-time PCR assay. The real-time PCR demonstrated the presence of M. viscosa DNA sequences in 88.1% of samples collected from 35 outbreaks of winter-ulcer in Norwegian fish farms. In contrast, standard culturing on blood agar identified M. viscosa in only 39.7% of fish. While the culturing method revealed a similar prevalence (26 to 27%) of M. viscosa in kidney and ulcer samples, substantially more ulcer (81.5%) than kidney (49.7%) samples were shown positive by real-time PCR.

Citations

Apr 5, 2011·Journal of Applied Microbiology·H BjörnssonE Benediktsdóttir
Aug 23, 2018·Journal of Fish Diseases·Thorbjorg EinarsdottirElisabet Einarsdottir
Apr 26, 2019·Diseases of Aquatic Organisms·Brett MacKinnonSophie St-Hilaire
Oct 22, 2020·Proceedings. Biological Sciences·Dylan SheaMartin Krkošek
Feb 22, 2021·Journal of Microbiological Methods·Roman NetzerTrisha Dhawan

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