Real-time PCR for Helicobacter pylori diagnosis. The best tools available

Helicobacter
Lucie BénéjatF Mégraud

Abstract

Knowledge of antimicrobial susceptibility, especially to macrolides, has become crucial for the management of Helicobacter pylori infection. Our aim was to evaluate two new PCR kits able to detect H. pylori in gastric biopsies as well as the mutations associated with macrolide resistance. Two hundred successive biopsies (received from gastroenterologists all over France) were used. The two new kits tested were Amplidiag H. pylori+ClariR from Mobidiag Espoo, Finland, and RIDA® GENE H. pylori from R-Biopharm, Darmstadt, Germany. Culture and a validated in-house real-time PCR were also performed, and in the case of a positive culture, Etest for clarithromycin was carried out. Discrepancies were solved by looking at the pathologic data. Culture was positive in 68 cases (34%), and with our in-house real-time PCR in these 68 cases plus 5 others (N = 73, 36%). All were also detected by the two new kits. In addition, RIDA® GENE H. pylori detected one more positive also detected by Amplidiag H. pylori+ClariR , and Amplidiag detected two other positives. Of these three additional cases, pathology confirmed the positivity for two. Only one case diagnosed by Amplidiag could be considered as a false positive. With regard to clarithromycin r...Continue Reading

References

Dec 1, 1985·Journal of Clinical Microbiology·F MegraudH Lamouliatte
Apr 1, 1997·Journal of Clinical Microbiology·L MonteiroF Mégraud
Feb 28, 2002·Alimentary Pharmacology & Therapeutics·P MalfertheinerUNKNOWN European Helicobacter Pylori Study Group (EHPSG)
Apr 13, 2007·Clinical Microbiology Reviews·Francis Mégraud, Philippe Lehours
Jun 28, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A DucournauF Mégraud
Nov 2, 2016·Gut·P MalfertheinerUNKNOWN European Helicobacter and Microbiota Study Group and Consensus panel

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Citations

Nov 30, 2018·Current Opinion in Gastroenterology·Sinéad M SmithDeirdre McNamara
Sep 6, 2019·Helicobacter·Athanasios MakristathisEmilie Bessède
Apr 11, 2021·Gut·Francis MegraudUNKNOWN European Helicobacter pylori Antimicrobial Susceptibility Testing Working Group
Jun 3, 2021·Journal of Clinical Medicine·Maria Pina Dore, Giovanni Mario Pes
Aug 15, 2021·Gastroenterology·Francis Mégraud, Emilie Bessède

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