Real-time PCR melting analysis with fiber optic SPR enables multiplex DNA identification of bacteria

The Analyst
D DaemsJeroen Lammertyn

Abstract

A fiber optic surface plasmon resonance (FO-SPR) technology was developed that enables simultaneous quantification and identification of multiple DNA targets on the same platform. The bioassay was based on the hybridization/melting of DNA-coated Au nanoparticles on the FO-SPR sensor when targets are present. The multiplex concept was successfully demonstrated on two related bacteria and for detection of multiple mutations in sequences. In conclusion, FO-SPR technology shows a great potential as a next generation in vitro diagnostics tool.

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·C T WittwerK S Elenitoba-Johnson
Mar 9, 2002·Nucleic Acids Research·Ian M MackayAndreas Nitsche
Feb 6, 2003·Journal of the American Chemical Society·Rongchao JinGeorge C Schatz
Apr 15, 2004·Nature Reviews. Microbiology·David MabeyMark D Perkins
May 7, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Lauren K WolfRosina M Georgiadis
Oct 3, 2006·Nucleic Acids Research·Anthony P MalanoskiDavid A Stenger
Dec 15, 2006·Analytical Chemistry·Sarah J HurstChad A Mirkin
Apr 3, 2007·Nature Protocols·Jesse MontgomeryLuming Zhou
Jun 15, 2007·Pharmacogenomics·Gudrun H ReedCarl T Wittwer
Nov 27, 2009·Nature·David A Giljohann, Chad A Mirkin
Dec 17, 2009·Nature Reviews. Microbiology·Sascha Sauer, Magdalena Kliem
Mar 8, 2011·Trends in Biotechnology·Angelika NiemzDavid S Boyle
Mar 12, 2011·Small·Jeroen PolletJeroen Lammertyn
Aug 7, 2012·Nature Reviews. Microbiology·Nicholas J LomanMark J Pallen
Sep 13, 2014·Biosensors & Bioelectronics·Karel KnezJeroen Lammertyn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.