Real-time PCR Tests in Dutch Exotic Mosquito Surveys; Implementation of Aedes aegypti and Aedes albopictus Identification Tests, and the Development of Tests for the Identification of Aedes atropalpus and Aedes japonicus japonicus (Diptera: Culicidae)

Journal of Medical Entomology
B T L H van de VossenbergE J Scholte

Abstract

Since 2009, The Netherlands Food and Consumer Product Safety Authority carries out surveys focusing on, amongst others, the presence of invasive mosquito species (IMS). Special attention is given to exotic container-breeding Aedes species Aedes aegypti (L.), Aedes albopictus (Skuse), Aedes atropalpus (Coquillett), and Aedes japonicus japonicus (Theobald). This study describes the implementation of real-time PCR tests described by Hill et al. (2008) for the identification of Ae. aegypti and Ae. albopictus, and the development of two novel real-time PCR tests for the identification of Ae. atropalpus and Ae. j. japonicus. Initial test showed that optimization of elements of the Ae. aegypti and Ae. albopictus tests was needed. Method validation tests were performed to determine if the implemented and newly developed tests are fit for routine diagnostics. Performance criteria of analytical sensitivity, analytical specificity, selectivity, repeatability, and reproducibility were determined. In addition, experiments were performed to determine the influence of environmental conditions on the usability of DNA extracted from mosquito specimens trapped in BG-Sentinel traps. The real-time PCR tests were demonstrated to be sensitive, speci...Continue Reading

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Citations

Jan 1, 2016·Journal of Medical Entomology·Ioannis A GiantsisMarie Claude Bon
Feb 9, 2019·Medical and Veterinary Entomology·C D SchenkelC Silaghi
May 21, 2020·International Journal of Environmental Research and Public Health·Adolfo Ibáñez-JusticiaArjan Stroo
Aug 1, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Adolfo Ibáñez-JusticiaArjan Stroo
Mar 2, 2021·Journal of the American Mosquito Control Association·Adolfo Ibáñez-JusticiaWillem Takken
Jul 28, 2021·Journal of Medical Entomology·Isra DeblauweWim Van Bortel
May 30, 2021·Transboundary and Emerging Diseases·Karin Bakran-LeblHans-Peter Fuehrer

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