Real-time, quantitative PCR assays for the detection of virus-specific DNA in samples with mixed populations of polyomaviruses

Journal of Virological Methods
Achintya PalAndrew M Lewis

Abstract

Mixtures of polyomaviruses can be present in the central nervous system, the gastrointestinal tract, the genitourinary tract, blood, and urban sewage. We have developed 12 primer/probe sets (four per virus) for real-time, quantitative PCR assays (TaqMan) that can specifically detect BKV, JCV, and SV40 genomes present in mixtures of these viruses. The specificities of these primer/probe sets were determined by evaluating their level of interaction with the DNA from other polyomaviruses and their ability to estimate the number of copies of homologous viral DNA in blinded samples of defined mixtures of three polyomaviral DNAs. Three early region and three late region primer/probe sets determined, within a two-fold range, the number of copies of their respective DNAs. Four sets of SV40 primer/probes also detected 1.1-2.4 copies of SV40 DNA per COS-1 cell, cells estimated to contain a single copy of SV40 DNA. Three JCV primer/probe sets detected 3.7-4.2 copies per cell of JCV DNA in the JCV-transformed cell line M1-HR, cells estimated to contain between 0.5 and 1 copy of the JCV genome. We suggest that the virus-specific primer/probe sets in this study be considered sufficiently characterized to initiate the quantification of polyom...Continue Reading

References

Apr 1, 1978·Journal of Clinical Pathology·D V ColemanW J Russell
May 11, 1978·Nature·W FiersM Ysebaert
Dec 1, 1979·Cell·I SeifR Dhar
Sep 19, 1980·Science·K W PedenD Nathans
Oct 1, 1980·The Journal of Infectious Diseases·W D SheffieldS S Kalter
Jan 1, 1982·Journal of Medical Virology·J GoudsmitJ van der Noordaa
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·L LaghiC R Boland
Feb 10, 2000·Human Pathology·J S ButelE Adam
Nov 29, 2001·Journal of Clinical Microbiology·D M WhileyT P Sloots
Mar 19, 2002·Lancet·Narayan ShivapurkarAdi F Gazdar
Sep 5, 2002·International Journal of Cancer. Journal International Du Cancer·Eric A EngelsKeerti V Shah
Dec 3, 2002·Nature Reviews. Cancer·Adi F GazdarMichele Carbone
Apr 15, 2003·Journal of Virology·Robert L Garcea, Michael J Imperiale
May 27, 2003·Journal of Medical Virology·P MinorW Knowles
Jun 10, 2003·International Journal of Cancer. Journal International Du Cancer·Michele CarboneAnthony Yeung
Jul 15, 2003·Journal of Medical Virology·Wendy A KnowlesElizabeth Miller
Oct 16, 2003·Journal of the National Cancer Institute·Joseph J CarterDenise A Galloway
Jan 14, 2005·Journal of Medical Virology·John A VanchiereJanet S Butel
Apr 7, 2005·Nature Medicine·Emily Singer
Apr 9, 2005·Nature Biotechnology·Cormac Sheridan
Aug 10, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Adrienne L McNeesJanet S Butel

❮ Previous
Next ❯

Citations

Oct 10, 2006·Applied and Environmental Microbiology·Silvia Bofill-MasRosina Girones
Jul 10, 2012·Applied and Environmental Microbiology·Adriana de Abreu CorrêaSílvia Bofill-Mas
Oct 6, 2011·MBio·Paul G CantalupoJames M Pipas
Sep 29, 2012·Emerging Infectious Diseases·Erica A SiebrasseDavid Wang
Dec 14, 2011·Journal of Visualized Experiments : JoVE·Silvia Bofill-MasRosina Girones
Nov 5, 2013·International Journal of Food Microbiology·Doris Sobral Marques SouzaCélia Regina Monte Barardi
Jan 20, 2016·Food and Environmental Virology·Carmen Baur VieiraMarize Pereira Miagostovich
Oct 17, 2015·Journal of Applied Microbiology·Y QiuXiaoli Pang
Nov 13, 2015·The Science of the Total Environment·Andri T RachmadiCharles P Gerba
Nov 1, 2011·Ecotoxicology and Environmental Safety·Doris Sobral Marques SouzaCélia Regina Monte Barardi
Dec 15, 2015·Reviews in Medical Virology·Martyn K WhiteKamel Khalili
Sep 5, 2009·Journal of Virological Methods·Haruhiko MurataKeith Peden
Jan 20, 2009·Journal of Medical Virology·Sarah J TozerTheo P Sloots
Sep 4, 2015·Infectious Agents and Cancer·Hector RodríguezFrancisco Aguayo
Nov 23, 2011·Marine Pollution Bulletin·V MorescoC R M Barardi
Jun 12, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Seweryn BialasiewiczTheo P Sloots
Nov 21, 2012·Journal of Virological Methods·Byron CalguaRosina Girones
Sep 13, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mireia SospedraRoland Martin
May 20, 2016·Environmental Science and Pollution Research International·X Fernandez-CassiS Bofill-Mas
Mar 24, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S BialasiewiczT P Sloots
Mar 31, 2017·Applied Microbiology and Biotechnology·María Dolores Blanco FernándezViviana Andrea Mbayed
Dec 4, 2014·Arquivos de neuro-psiquiatria·Luiz Henrique da Silva NaliAugusto Cesar Penalva de Oliveira
Jun 27, 2017·Oral Diseases·M A FigueiredoK L Ortega
Aug 4, 2018·Transplant Infectious Disease : an Official Journal of the Transplantation Society·Camila Silva BicalhoLigia Camera Pierrotti
Jan 24, 2019·Journal of Applied Oral Science : Revista FOB·Dmitry José de Santana SarmentoPaulo Henrique Braz-Silva
Jan 27, 2019·Journal of Medical Virology·Paulo Roberto P UrbanoCamila M Romano
Apr 5, 2019·Food and Environmental Virology·Viviana BortagarayMatías Victoria

❮ Previous
Next ❯

Related Concepts

Related Feeds

BK Virus Infection

BK virus infection is a significant complication of modern immunosuppression used in kidney transplantation. Discover the latest research on BK virus infection here.

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.