Real-time quantitative PCR measurement of thyroglobulin mRNA in peripheral blood of thyroid cancer patients and healthy subjects

Annals of the New York Academy of Sciences
Diane LiR Swaminathana

Abstract

Follow-up of recurrent differentiated thyroid carcinoma involves the measurement of serum thyroglobulin (Tg). However, Tg autoantibodies are present in a high proportion of thyroid carcinoma patients (up to 25%) and these can interfere with the Tg immunoassays. To overcome this obstacle, investigators have used real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) to measure Tg mRNA in the blood of patients with differentiated thyroid cancer, with varying degrees of success. In the present study, we demonstrate the first reported use of the PAXgene Blood RNA collection tube and extraction kit method for the preparation of RT-PCR-quality RNA with subsequent deployment of the latter in the development of a specific, sensitive, and reproducible Taqman assay for the detection and quantification of thyroglobulin mRNA. Beta-actin mRNA was also assayed and results are expressed as a ratio of Tg to beta-actin mRNA. The intra-assay coefficient of variations (CVs) for Tg and beta-actin mRNA assay were 27.7% and 25.4%, respectively. Inter-assay CVs were 20.8% and 28.8%, respectively, for the two assays. Tg mRNA was detected in all cancer subjects (n = 42) and healthy individuals (n = 20). Tg mRNA was significantl...Continue Reading

References

Mar 11, 2000·The Journal of Laboratory and Clinical Medicine·S J McFaulV M Villa

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Citations

Sep 30, 2005·Virchows Archiv : an International Journal of Pathology·Giuseppe PelosiGiuseppe Viale
Jan 31, 2007·Thyroid : Official Journal of the American Thyroid Association·David C Howlett
Jan 20, 2006·Thyroid : Official Journal of the American Thyroid Association·David S CooperUNKNOWN American Thyroid Association Guidelines Taskforce
Oct 29, 2009·Thyroid : Official Journal of the American Thyroid Association·UNKNOWN American Thyroid Association (ATA) Guidelines Taskforce on Thyroid Nodules and Differentiated Thyroid CancerR Michael Tuttle
Jul 31, 2010·Journal of Oncology·Claudia PapewalisMatthias Schott
Aug 3, 2006·Surgical Oncology Clinics of North America·Julie Ann Sosa, Robert Udelsman
Oct 8, 2008·Annals of the New York Academy of Sciences·N C HolfordR Swaminathan
Oct 8, 2008·Annals of the New York Academy of Sciences·Amy WongR Swaminathan
Feb 22, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Reidun ØvstebøKari Bente Foss Haug
Sep 1, 2016·Endocrine-related Cancer·Benjamin C ThomasHayley C Whitaker
Jun 5, 2012·Toxicologic Pathology·Igor MikaelianBruce E Leroy
Oct 8, 2008·Annals of the New York Academy of Sciences·S AgalouR Swaminathan
Nov 22, 2005·Clinical Chemistry·Emanuela OrlandiR Swaminathan
Aug 29, 2006·Arquivos brasileiros de endocrinologia e metabologia·Sabrina Mendes CoelhoDenise Pires de Carvalho

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