PMID: 11345132May 10, 2001Paper

Real-time quantitative PCR of telomerase mRNA is useful for the differentiation of benign and malignant pancreatic disorders

Pancreas
P BüchlerH Friess

Abstract

The presence of telomerase activity has been proposed as a specific and sensitive marker for malignant tissue, and positivity rates of up to 95% have been reported in pancreatic cancer. In the present study telomerase activity analysis was reevaluated in 29 pancreatic cancer tissues compared with 36 chronic pancreatitis tissues and 21 normal controls, and a study was made of whether malignant and benign pancreatic disorders can be better differentiated using a novel technique real-time quantitative PCR analysis-analyzing telomerase mRNA expression. Telomerase activity was present in 35% (10 of 29) of pancreatic cancer samples, 3% (one of 36) of chronic pancreatitis samples, and none of the normal pancreatic tissue samples in the TRAP assay. Real-time quantitative PCR analysis revealed the presence of telomerase mRNA expression in 50% (10 of 20) of normal, 86% (31 of 36) of chronic pancreatitis, and 90% (26 of 29) of pancreatic cancer samples. However, quantification of the expression data revealed that the relative increase above normal was 5.5 (range, 3.5-8.6) for chronic pancreatitis and 23.9 (range, 18.6-30.7) for pancreatic cancer samples (p < 0.01). No relationship was found between telomerase activity and the fold increas...Continue Reading

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Citations

Mar 13, 2003·Current Gastroenterology Reports·Friedrich H Schmitz-WinnenthalMarkus W Büchler
Jul 28, 2004·Gastrointestinal Endoscopy·Richard A Erickson
Nov 28, 2001·Drug Discovery Today·Michel M. Ouellette, Kwangmoon Lee
Oct 7, 2003·Annual Review of Genomics and Human Genetics·Donna E HanselRalph H Hruban
Apr 20, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nadia ZaffaroniMaria Grazia Daidone
Aug 6, 2010·PloS One·Siew-Kee LowHiromi Sakamoto
Nov 6, 2004·Gastroenterology Clinics of North America·Asif KhalidKevin McGrath
Mar 8, 2005·Clinics in Laboratory Medicine·Asif KhalidKevin McGrath
Jun 18, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Antonio MarchettiSilvano Bosari

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