Real-Time Tracking of Ex Vivo -Expanded Natural Killer Cells Toward Human Triple-Negative Breast Cancers

Frontiers in Immunology
Tung Nguyen Thanh UongMee Sun Yoon

Abstract

Ex vivo-expanded natural killer (NK) cells are a potential candidate for cancer immunotherapy based on high cytotoxicity against malignant tumor cells. However, a limited understanding of the migration of activated NK cells toward solid tumors is a critical dilemma in the development of effective and adoptive NK cell-based immunotherapy. Ex vivo-expanded NK cells from healthy donors were stained with near-infrared fluorophores at different concentrations. NK cell proliferation and cytotoxicity were assessed using a WST-8 assay, while the expression levels of surface molecules were analyzed by flow cytometry. To investigate the biodistribution of NK cells in both normal and tumor-bearing NSG mice, NK cells labeled with ESNF13 were subjected to NIR fluorescence imaging using the Mini-FLARE imaging system. Finally, mice were sacrificed and histopathological tests were performed in resected organs. The signal intensity of ESNF-stained NK cells was long-lasting at 72 h using concentrations as low as 0.04 µM. At a low dose range, ESNF13 did not affect NK cell purity, expression levels of surface receptors, or cytotoxic functions against MDA-MB-231 cancer cells. Ex vivo-expanded NK cells labeled with ESNF13 had a 4-h biodistribution i...Continue Reading

References

Dec 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Domingo F Barber, Eric O Long
Dec 20, 2003·European Journal of Nuclear Medicine and Molecular Imaging·Birgit MellerManfred Baehre
Jul 1, 2008·Nuclear Medicine and Biology·Reinhard MeierHeike E Daldrup-Link
Apr 23, 2009·Cancer Research·Hiroyuki FujisakiDario Campana
Jun 15, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Duck ChoDario Campana
Aug 13, 2010·Cancer Immunology, Immunotherapy : CII·Eleni G IliopoulouSonia A Perez
Jun 5, 2012·The New England Journal of Medicine·Suzanne L TopalianMario Sznol
Jan 29, 2013·Biomedical Materials·Soon Hee KimHak Soo Choi
Mar 26, 2013·Cellular & Molecular Immunology·Isabela Pedroza-PachecoAurore Saudemont
Feb 12, 2015·Oncotarget·Renaud SabatierFrançois Bertucci
Mar 11, 2015·Nature·Christina Twyman-Saint VictorAndy J Minn
Jun 20, 2015·Frontiers in Immunology·Okjae LimEui-Cheol Shin
May 4, 2016·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Rita NandaLaurence Buisseret
Jul 6, 2016·Frontiers in Immunology·Iona S SchusterMariapia A Degli-Esposti
Jul 26, 2016·Frontiers in Immunology·Katharina Pallmer, Annette Oxenius
Sep 24, 2016·PloS One·Madhavi PuchalapalliJennifer E Koblinski
Jun 7, 2017·Chonnam Medical Journal·Danbi Jo, Hoon Hyun
Jan 15, 2018·Journal of Proteomics·Dmitri V RozanovPaul T Spellman

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Citations

Jul 22, 2019·Cancers·Michela VaraniFilippo Galli
Feb 23, 2019·Journal of Neuro-oncology·Bridget A KennisVidya Gopalakrishnan
Mar 3, 2020·Frontiers in Medicine·Justine PerrinJacques Barbet
Oct 21, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Yufeng WangXiaozhe Han
Jan 20, 2021·ACS Biomaterials Science & Engineering·Madison N TemplesBlanka Sharma

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Methods Mentioned

BETA
xenograft
imaging techniques
fluorescence imaging
flow cytometry
enzyme-linked immunosorbent assay
xenografts
genetic modification
PCR

Clinical Trials Mentioned

NCT03125902
NCT03197935

Software Mentioned

SPSS
Elements Basic Research
NIS
CellQuest Pro
WinMDI
BD

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